PMID- 9201036
OWN - NLM
STAT- MEDLINE
DCOM- 19970711
LR  - 20190706
IS  - 0009-7330 (Print)
IS  - 0009-7330 (Linking)
VI  - 81
IP  - 1
DP  - 1997 Jul
TI  - Regional localization of ERG, the channel protein responsible for the rapid 
      component of the delayed rectifier, K+ current in the ferret heart.
PG  - 128-35
AB  - Repolarization of the cardiac action potential varies widely throughout the 
      heart. This could be due to the differential distribution of ion channels 
      responsible for repolarization, especially the K+ channels. We have therefore 
      studied the cardiac localization of ERG, a channel protein known to play an 
      important role in generation of the rapid component of the delayed rectifier K+ 
      current (IKr), an important determinant of the repolarization waveform, 
      Cryosections of the ferret atrium and ventricle were prepared to determine the 
      localization of ERG by fluorescence in situ hybridization (FISH) and 
      immunofluorescence. We found that in the ferret, ERG transcript and protein 
      expression was most abundant in the epicardial cell layers throughout most of the 
      ventricle, except at the base. In the atrium, we found that ERG is most abundant 
      in the medial right atrium, especially in the trabeculae and the crista 
      terminalis of the right atrial appendage. It also is present in areas within the 
      sinoatrial node. In all regions studied, FISH and immunofluorescence showed 
      concordant localization patterns. These data suggest that repolarization mediated 
      by IKr is not uniform throughout the ferret heart and provide a molecular 
      explanation for heterogeneity in action potential repolarization throughout the 
      mammalian heart.
FAU - Brahmajothi, M V
AU  - Brahmajothi MV
AD  - Department of Medicine, Duke University School of Medicine, Durham, NC 27710, 
      USA.
FAU - Morales, M J
AU  - Morales MJ
FAU - Reimer, K A
AU  - Reimer KA
FAU - Strauss, H C
AU  - Strauss HC
LA  - eng
GR  - BIR-9318118/PHS HHS/United States
GR  - HL-19216/HL/NHLBI NIH HHS/United States
PT  - Journal Article
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - United States
TA  - Circ Res
JT  - Circulation research
JID - 0047103
RN  - 0 (Antibodies)
RN  - 0 (Potassium Channels)
RN  - 0 (Proteins)
SB  - IM
EIN - Circ Res 1997 Sep;81(3):455
MH  - Action Potentials
MH  - Animals
MH  - Antibodies/analysis
MH  - Enzyme-Linked Immunosorbent Assay
MH  - *Ferrets
MH  - Fluorescent Antibody Technique
MH  - *Genes/immunology/physiology
MH  - Heart/*physiology
MH  - Heart Atria/cytology/metabolism
MH  - Heart Ventricles/cytology/metabolism
MH  - In Situ Hybridization, Fluorescence
MH  - In Vitro Techniques
MH  - Myocardium/cytology/*metabolism
MH  - Potassium Channels/*analysis/*genetics/physiology
MH  - Proteins/*analysis
EDAT- 1997/07/01 00:00
MHDA- 1997/07/01 00:01
CRDT- 1997/07/01 00:00
PHST- 1997/07/01 00:00 [pubmed]
PHST- 1997/07/01 00:01 [medline]
PHST- 1997/07/01 00:00 [entrez]
AID - 10.1161/01.res.81.1.128 [doi]
PST - ppublish
SO  - Circ Res. 1997 Jul;81(1):128-35. doi: 10.1161/01.res.81.1.128.