PMID- 9201257
OWN - NLM
STAT- MEDLINE
DCOM- 19970721
LR  - 20191210
IS  - 0741-5400 (Print)
IS  - 0741-5400 (Linking)
VI  - 61
IP  - 6
DP  - 1997 Jun
TI  - Expression of TNF-alpha by human plasma cells in chronic inflammation.
PG  - 667-78
AB  - Tumor necrosis factor-alpha (TNF-alpha) is a potent pro-inflammatory cytokine and 
      mediator of the inflammatory response. It has been implicated in the pathogenesis 
      of many inflammatory disorders, including rheumatoid arthritis (RA), septic 
      shock, and Crohn's disease. Using a specific anti-human TNF-alpha antibody we 
      detected immunoreactivity for this cytokine in the cytoplasm of inflammatory 
      cells in several chronic inflammatory disorders, including RA, scleritis, and 
      polyarteritis nodosa. These cells were identified predominantly as IgG-expressing 
      plasma cells. Lymph nodes from patients with Hodgkin's lymphoma and breast 
      cancer, but not from control subjects, were also found to contain 
      TNF-alpha-positive plasma cells. Cultured EBV-B lymphocytes and a human plasma 
      cell line (ARH-77) when stimulated with phorbol myristate acetate demonstrated 
      cytoplasmic TNF-alpha immunoreactivity. Western blot analysis of cell membranes 
      and conditioned media from both cell types revealed the presence of the 26-kDa 
      membrane-bound from and the 17-kDa soluble from of TNF-alpha, respectively. 
      TNF-alpha was quantitated by enzyme-linked immunosorbent assay and found to be 
      biologically active as determined by the L929 cytotoxicity assay. This is the 
      first demonstration that plasma cells may be capable of modulating immune and 
      inflammatory responses, not only by antibody production, but also by their 
      secretion of a key inflammatory mediator, TNF-alpha.
FAU - Di Girolamo, N
AU  - Di Girolamo N
AD  - Inflammation Research Unit, School of Pathology, University of New South Wales, 
      Sydney, Australia.
FAU - Visvanathan, K
AU  - Visvanathan K
FAU - Lloyd, A
AU  - Lloyd A
FAU - Wakefield, D
AU  - Wakefield D
LA  - eng
PT  - Journal Article
PL  - England
TA  - J Leukoc Biol
JT  - Journal of leukocyte biology
JID - 8405628
RN  - 0 (Culture Media, Conditioned)
RN  - 0 (Tumor Necrosis Factor-alpha)
RN  - NI40JAQ945 (Tetradecanoylphorbol Acetate)
SB  - IM
MH  - Animals
MH  - Blotting, Western
MH  - Cells, Cultured
MH  - Chronic Disease
MH  - Culture Media, Conditioned
MH  - Enzyme-Linked Immunosorbent Assay
MH  - Humans
MH  - Inflammation/*immunology/metabolism/*pathology
MH  - L Cells
MH  - Lymph Nodes/immunology/pathology
MH  - Lymphocyte Activation/drug effects
MH  - Mice
MH  - Plasma Cells/drug effects/immunology/*metabolism
MH  - Solubility
MH  - Tetradecanoylphorbol Acetate/pharmacology
MH  - Tumor Necrosis Factor-alpha/*biosynthesis/immunology
EDAT- 1997/06/01 00:00
MHDA- 1997/06/01 00:01
CRDT- 1997/06/01 00:00
PHST- 1997/06/01 00:00 [pubmed]
PHST- 1997/06/01 00:01 [medline]
PHST- 1997/06/01 00:00 [entrez]
AID - 10.1002/jlb.61.6.667 [doi]
PST - ppublish
SO  - J Leukoc Biol. 1997 Jun;61(6):667-78. doi: 10.1002/jlb.61.6.667.