PMID- 9201261
OWN - NLM
STAT- MEDLINE
DCOM- 19970721
LR  - 20190516
IS  - 0741-5400 (Print)
IS  - 0741-5400 (Linking)
VI  - 61
IP  - 6
DP  - 1997 Jun
TI  - Complex regulation of human neutrophil activation by actin filaments: 
      dihydrocytochalasin B and botulinum C2 toxin uncover the existence of multiple 
      cation entry pathways.
PG  - 703-11
AB  - In human neutrophils, the chemotactic peptide, 
      N-formyl-L-methionyl-L-leucyl-L-phenalalanine (fMLP), the Ca(2+)-ATPase 
      inhibitor, thapsigargin, and the lectins, concanavalin A (Con A) and mistletoe 
      lectin I (ML I), stimulate the entry of Ca2+ and Na+ with subsequent activation 
      of exocytosis and superoxide anion (O2-) formation. We studied the role of actin 
      in neutrophil activation. The actin filament-disrupting substances, 
      dihydrocytochalasin B (dhCB) and botulinum C2 toxin (C2 toxin) potentiated fMLP- 
      and lectin-stimulated Ca(2+)- and Na+ entry. Lectin-induced Mn2+ entry was 
      enhanced by actin disruption, whereas fMLP-triggered Mn2+ entry was unaffected. 
      dhCB and C2 toxin inhibited fMLP- and lectin-stimulated Ba2+ influx. The actin 
      disrupters also inhibited fMLP- and ML I-induced Sr2+ influx, whereas Con 
      A-stimulated Sr2+ entry was not influenced by dhCB and C2 toxin. 
      Thapsigargin-stimulated cation entry was not altered by actin disruption. DhCB 
      and botulinum C2 toxin potentiated lysozyme release induced by all four stimuli. 
      Con A and ML I per se activated O2- formation only in the presence and not in the 
      absence of dhCB. Con A potentiated the stimulatory effects of ML I on O2- 
      formation in the presence of dhCB and primed neutrophils to respond to ML I in 
      the absence of dhCB. Our data indicate the following: (1) dhCB and C2 toxin 
      uncover the existence of multiple cation entry pathways in neutrophils; (2) actin 
      disruption facilitates exocytosis and O2- formation by enhancement of Ca(2+)- and 
      Na+ entry and by altering the function of proteins involved in activation of 
      secretion and O2- formation; and (3) Con A and ML I, which possess different 
      sugar specificities, activate different signaling pathways in neutrophils.
FAU - Wenzel-Seifert, K
AU  - Wenzel-Seifert K
AD  - Institut fur Pharmakologie, Freie Universitat Berlin, Germany.
FAU - Lentzen, H
AU  - Lentzen H
FAU - Aktories, K
AU  - Aktories K
FAU - Seifert, R
AU  - Seifert R
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - England
TA  - J Leukoc Biol
JT  - Journal of leukocyte biology
JID - 8405628
RN  - 0 (Actins)
RN  - 0 (Cations)
RN  - 0 (Lectins)
RN  - 0 (Plant Preparations)
RN  - 0 (Plant Proteins)
RN  - 0 (Ribosome Inactivating Proteins, Type 2)
RN  - 0 (Toxins, Biological)
RN  - 0 (mistletoe lectin I)
RN  - 0 (ribosome inactivating protein, Viscum)
RN  - 11062-77-4 (Superoxides)
RN  - 24GP945V5T (Barium)
RN  - 39156-67-7 (dihydrocytochalasin B)
RN  - 3CHI920QS7 (Cytochalasin B)
RN  - 42Z2K6ZL8P (Manganese)
RN  - 59880-97-6 (N-Formylmethionine Leucyl-Phenylalanine)
RN  - 67526-95-8 (Thapsigargin)
RN  - 9NEZ333N27 (Sodium)
RN  - EC 3.2.1.17 (Muramidase)
RN  - EC 3.4.24.69 (Botulinum Toxins)
RN  - FPM7829VMX (botulinum toxin type C)
RN  - SY7Q814VUP (Calcium)
RN  - YZS2RPE8LE (Strontium)
SB  - IM
MH  - Actins/blood/drug effects/*physiology
MH  - Barium/blood
MH  - Botulinum Toxins/*pharmacology
MH  - Calcium/blood
MH  - Cations/*blood
MH  - Cytochalasin B/*analogs & derivatives/pharmacology
MH  - Female
MH  - Humans
MH  - Ion Transport/drug effects
MH  - Lectins/pharmacology
MH  - Male
MH  - Manganese/blood
MH  - Muramidase/blood
MH  - N-Formylmethionine Leucyl-Phenylalanine/pharmacology
MH  - Neutrophil Activation/*drug effects
MH  - Neutrophils/drug effects/*metabolism
MH  - *Plant Preparations
MH  - *Plant Proteins
MH  - Ribosome Inactivating Proteins, Type 2
MH  - Sodium/blood
MH  - Strontium/blood
MH  - Superoxides/blood
MH  - Thapsigargin/pharmacology
MH  - Toxins, Biological/pharmacology
EDAT- 1997/06/01 00:00
MHDA- 1997/06/01 00:01
CRDT- 1997/06/01 00:00
PHST- 1997/06/01 00:00 [pubmed]
PHST- 1997/06/01 00:01 [medline]
PHST- 1997/06/01 00:00 [entrez]
AID - 10.1002/jlb.61.6.703 [doi]
PST - ppublish
SO  - J Leukoc Biol. 1997 Jun;61(6):703-11. doi: 10.1002/jlb.61.6.703.