PMID- 9202203
OWN - NLM
STAT- MEDLINE
DCOM- 19970724
LR  - 20141120
IS  - 0013-7227 (Print)
IS  - 0013-7227 (Linking)
VI  - 138
IP  - 7
DP  - 1997 Jul
TI  - Acute and chronic exposure to tumor necrosis factor-alpha fails to affect 
      insulin-stimulated glucose metabolism of isolated rat soleus muscle.
PG  - 2674-9
AB  - To better understand the effects of tumor necrosis factor-alpha (TNF alpha) on 
      insulin sensitivity, direct interaction of the peptide with freshly isolated rat 
      soleus muscle strips was investigated. Muscles were exposed to TNF alpha at 
      concentrations ranging from 0.01-5 nmol/liter. Rates of insulin-stimulated (5 or 
      100 nmol/liter) glucose metabolism were determined after periods of TNF alpha 
      preexposure of 30 min, 6 h, and 24 h. Independent of exposure time, TNF alpha 
      failed to exert any significant effect on rates of 3H-2-deoxy-glucose transport 
      (stimulation by 100 nmol/liter insulin after preincubation without vs. with 5 
      nmol/liter TNF alpha, cpm/mg x h: 30 min, 779 +/- 29 vs. 725 +/- 29; 6 h, 652 +/- 
      56 vs. 617 +/- 60; 24 h, 911 +/- 47 vs. 936 +/- 31) or glucose incorporation into 
      glycogen (micromol/g x h: 30 min, 5.19 +/- 0.22 vs. 5.25 +/- 0.41; 6 h, 2.08 +/- 
      0.10 vs. 2.09 +/- 0.17; 24 h, 2.51 +/- 0.21 vs. 2.41 +/- 0.26). In parallel, TNF 
      alpha neither affected insulin-stimulated rates of glucose oxidation (CO2 
      production) and anaerobic glycolysis (lactate release), nor muscle glycogen 
      content. In conclusion, these findings do not support the hypothesis of muscle 
      insulin desensitization by TNF alpha via autocrine or paracrine mechanisms. The 
      obtained data favor the concept that TNF alpha-dependent muscle insulin 
      resistance in vivo depends on indirect effects rather than direct interaction of 
      the peptide with skeletal muscle.
FAU - Furnsinn, C
AU  - Furnsinn C
AD  - Department of Medicine III, University of Vienna, Austria. 
      clemens.fuernsinn@akh-wien.ac.at
FAU - Neschen, S
AU  - Neschen S
FAU - Wagner, O
AU  - Wagner O
FAU - Roden, M
AU  - Roden M
FAU - Bisschop, M
AU  - Bisschop M
FAU - Waldhausl, W
AU  - Waldhausl W
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - Endocrinology
JT  - Endocrinology
JID - 0375040
RN  - 0 (Hypoglycemic Agents)
RN  - 0 (Insulin)
RN  - 0 (Recombinant Proteins)
RN  - 0 (Tumor Necrosis Factor-alpha)
RN  - IY9XDZ35W2 (Glucose)
SB  - IM
MH  - Animals
MH  - Glucose/*metabolism
MH  - Hypoglycemic Agents/*pharmacology
MH  - In Vitro Techniques
MH  - Insulin/*pharmacology
MH  - Insulin Resistance
MH  - Male
MH  - Muscle, Skeletal/drug effects/*metabolism
MH  - Rats
MH  - Rats, Sprague-Dawley
MH  - Recombinant Proteins/pharmacology
MH  - Time Factors
MH  - Tumor Necrosis Factor-alpha/*pharmacology
EDAT- 1997/07/01 00:00
MHDA- 1997/07/01 00:01
CRDT- 1997/07/01 00:00
PHST- 1997/07/01 00:00 [pubmed]
PHST- 1997/07/01 00:01 [medline]
PHST- 1997/07/01 00:00 [entrez]
AID - 10.1210/endo.138.7.5219 [doi]
PST - ppublish
SO  - Endocrinology. 1997 Jul;138(7):2674-9. doi: 10.1210/endo.138.7.5219.