PMID- 9203094
OWN - NLM
STAT- MEDLINE
DCOM- 19970717
LR  - 20061115
IS  - 0022-3492 (Print)
IS  - 0022-3492 (Linking)
VI  - 68
IP  - 6
DP  - 1997 Jun
TI  - Effects of interleukin-1 beta on matrix metalloproteinase-3 levels in human 
      periodontal ligament cells.
PG  - 517-23
AB  - MATRIX METALLOPROTEINASE-3 (MMP-3), or stromelysin-1, is an enzyme responsible 
      for the degradation of a wide range of extracellular matrix proteins. Increases 
      in MMP-3 activity have been found in several chronic inflammatory diseases, and 
      this increased activity is thought to be mediated by interleukin-1 beta (IL-1 
      beta). Because IL-1 beta has been strongly associated with inflammatory 
      periodontal disease, the purpose of this in vitro study was to investigate the 
      role of IL-1 beta on the regulation of MMP-3 levels in cells derived from the 
      human periodontal ligament (PDL). Human PDL cell cultures were treated with IL-1 
      beta at varying concentrations (0.01-1.0 ng/ml) for 24 hour prior to analysis at 
      either transcript or protein levels. Following the isolation of total RNA, the 
      relative levels of MMP-3 mRNA were determined using reverse 
      transcription-polymerase chain reaction (RT-PCR) with 32P-end-labeled primers. 
      Immunocytochemical detection of MMP-3 protein was performed using polyclonal 
      antibodies to human MMP-3. The results of RT-PCR analysis demonstrated a 
      concentration-dependent increase in MMP-3 mRNA expression, with IL-1 beta 
      treatments of 0.1 and 1.0 ng/ml significantly (P < 0.01) increased over those 
      cells not treated with IL-1 beta. This increase in mRNA expression was paralleled 
      by significant (P < 0.001) changes at the protein level, with an average of 27.6% 
      of the cells stained positive for MMP-3 following IL-1 beta treatment (1.0 
      ng/ml), compared with control cells showing no positive staining for MMP-3. In 
      conclusion, the results of this study demonstrate that IL-1 beta upregulates 
      MMP-3 in human PDL cells on both an mRNA and a protein level. These findings 
      suggest possibly important roles for IL-1 beta and MMP-3 in both normal turnover 
      and maintenance of the PDL and in the connective tissue degradation associated 
      with periodontal disease.
FAU - Nakaya, H
AU  - Nakaya H
AD  - Department of Periodontics, University of Texas Health Science Center at San 
      Antonio, USA.
FAU - Oates, T W
AU  - Oates TW
FAU - Hoang, A M
AU  - Hoang AM
FAU - Kamoi, K
AU  - Kamoi K
FAU - Cochran, D L
AU  - Cochran DL
LA  - eng
PT  - Journal Article
PL  - United States
TA  - J Periodontol
JT  - Journal of periodontology
JID - 8000345
RN  - 0 (Extracellular Matrix Proteins)
RN  - 0 (Interleukin-1)
RN  - 0 (RNA, Messenger)
RN  - 0 (Recombinant Proteins)
RN  - EC 3.4.24.17 (Matrix Metalloproteinase 3)
SB  - IM
MH  - Analysis of Variance
MH  - Cells, Cultured
MH  - Enzyme Induction/drug effects/physiology
MH  - Extracellular Matrix Proteins/metabolism
MH  - Fibroblasts/drug effects/enzymology
MH  - Humans
MH  - Immunoenzyme Techniques
MH  - Interleukin-1/*pharmacology/*physiology
MH  - Matrix Metalloproteinase 3/*biosynthesis
MH  - Periodontal Ligament/cytology/drug effects/*enzymology
MH  - Periodontitis/enzymology
MH  - Polymerase Chain Reaction/methods
MH  - RNA, Messenger/analysis
MH  - Recombinant Proteins/pharmacology
MH  - Statistics, Nonparametric
MH  - Up-Regulation
EDAT- 1997/06/01 00:00
MHDA- 1997/06/01 00:01
CRDT- 1997/06/01 00:00
PHST- 1997/06/01 00:00 [pubmed]
PHST- 1997/06/01 00:01 [medline]
PHST- 1997/06/01 00:00 [entrez]
AID - 10.1902/jop.1997.68.6.517 [doi]
PST - ppublish
SO  - J Periodontol. 1997 Jun;68(6):517-23. doi: 10.1902/jop.1997.68.6.517.