PMID- 9204976
OWN - NLM
STAT- MEDLINE
DCOM- 19970717
LR  - 20190914
IS  - 0887-6924 (Print)
IS  - 0887-6924 (Linking)
VI  - 11
IP  - 7
DP  - 1997 Jul
TI  - Detection of 16 p deletions by FISH in patients with inv(16) or t(16;16) and 
      acute myeloid leukemia (AML).
PG  - 964-70
AB  - Deletions of sequences centromeric to the p-arm breakpoint have been described in 
      a subset of patients with inv(16) and acute myeloid leukemia (AML) and reported 
      to be associated with a relatively good prognosis. We have investigated 16 p 
      deletions in a cohort of 15 patients with AML and inv(16) or t(16;16) and 
      compared non-deletion and deletion patients in terms of clinical course. Patients 
      were studied by fluorescence in situ hybridization (FISH) using cosmid zit14 as a 
      probe to detect the presence of 16 p deletions in metaphase chromosomes of 
      leukemic cells. While seven patients (47%) revealed no evidence of a deletion, 
      five patients (33%) presented 16 p deletions, thus bringing further support to 
      the relatively frequent occurrence of this event in inv(16) patients. Remarkably, 
      two patients with inv(16) and one patient with t(16;16) showed a mosaicism of 
      deletion and non-deletion metaphases suggesting the presence of two distinct 
      leukemic cell populations. Results let us assume that 16 p deletions are not 
      restricted to inv(16) and may occur subsequently to inv(16) or t(16;16). The 
      presence of a 16 p deletion in a case of inv(16) associated with CBFB-MYH11 
      transcript type E indicates that deletions are not limited to CBFB-MYH11 
      transcript type A rearrangements. Survival of deletion patients was compared with 
      that of non-deletion and mosaic ones. No significant differences were observed. 
      The advantage of FISH for enumerative and quantitative assessment of 
      submicroscopic rearrangements of clinical significance is further emphasized.
FAU - Martinet, D
AU  - Martinet D
AD  - Division Autonome de Genetique Medicale, Centre Hospitalier Universitaire Vaudois 
      (CHUV), Lausanne, Switzerland.
FAU - Muhlematter, D
AU  - Muhlematter D
FAU - Leeman, M
AU  - Leeman M
FAU - Parlier, V
AU  - Parlier V
FAU - Hess, U
AU  - Hess U
FAU - Gmur, J
AU  - Gmur J
FAU - Jotterand, M
AU  - Jotterand M
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - England
TA  - Leukemia
JT  - Leukemia
JID - 8704895
SB  - IM
MH  - Adolescent
MH  - Adult
MH  - Aged
MH  - *Chromosome Deletion
MH  - *Chromosome Inversion
MH  - *Chromosomes, Human, Pair 16
MH  - Female
MH  - Humans
MH  - *In Situ Hybridization, Fluorescence
MH  - Leukemia, Myeloid, Acute/*genetics
MH  - Male
MH  - Middle Aged
MH  - *Translocation, Genetic
EDAT- 1997/07/01 00:00
MHDA- 1997/07/01 00:01
CRDT- 1997/07/01 00:00
PHST- 1997/07/01 00:00 [pubmed]
PHST- 1997/07/01 00:01 [medline]
PHST- 1997/07/01 00:00 [entrez]
AID - 10.1038/sj.leu.2400681 [doi]
PST - ppublish
SO  - Leukemia. 1997 Jul;11(7):964-70. doi: 10.1038/sj.leu.2400681.