PMID- 9204987
OWN - NLM
STAT- MEDLINE
DCOM- 19970717
LR  - 20130304
IS  - 0887-6924 (Print)
IS  - 0887-6924 (Linking)
VI  - 11
IP  - 7
DP  - 1997 Jul
TI  - Involvement of peripheral blood cells in multiple myeloma: chromosome changes are 
      the rule within circulating plasma cells but not within B lymphocytes.
PG  - 1034-9
AB  - The mononuclear cells in the peripheral blood are implicated in the myeloma 
      process especially with the presence of peripheral blood plasma cells (PBPC) and 
      clonal B lymphocytes found using phenotypic or gene rearrangement techniques. The 
      purpose of this study was to look for aneuploidy in the two main B cell 
      components of the peripheral blood: PBPC and CD20-positive B lymphocytes. 
      Conventional cytogenetics (CC) or DNA content analysis and fluorescence in situ 
      hybridization (FISH) with centromeric probes were performed on bone marrow plasma 
      cells (BMPC) of 21 patients with multiple myeloma and peripheral blood cells were 
      studied as follows: immunostaining to look for PBPC and to assess their number, 
      image analysis cytometry for the determination of their DNA content, and FISH 
      chromosomes analysis. FISH was performed using probes against the chromsomes that 
      were lost or gained in BMPC and was coupled with immunostaining of the relevant 
      light chain or CD20 antigen to study PBPC or B lymphocytes, respectively. 
      Monotypic PBPC were found in 16 patients. Their DNA content was the same or 
      nearly the same as for BMPC and they exhibited the same monosomies or trisomies 
      as those found within their BM counterpart. By contrast, DNA content of 
      mononuclear cells other than PBPC was within normal ranges, and in 13 of 15 
      patients CD20-positive B lymphocytes failed to show chromosomal changes by FISH 
      analysis. In two patients however, a few CD20+ cells with lymphoid morphology 
      exhibited chromosome changes, hypothesizing that a few cytogenetically abnormal B 
      cells without plasmocytic morphology may circulate. From these data, we conclude 
      that PBPC share the same genetic abnormalities as BMPC and thus belong to the 
      malignant clone, whereas most peripheral blood B lymphocytes are unrelated to the 
      tumor clone.
FAU - Zandecki, M
AU  - Zandecki M
AD  - Laboratoire d'Hematologie A, Hopital Calmette, CHU, Lille, France.
FAU - Bernardi, F
AU  - Bernardi F
FAU - Genevieve, F
AU  - Genevieve F
FAU - Lai, J L
AU  - Lai JL
FAU - Preudhomme, C
AU  - Preudhomme C
FAU - Flactif, M
AU  - Flactif M
FAU - Cosson, A
AU  - Cosson A
FAU - Bauters, F
AU  - Bauters F
FAU - Facon, T
AU  - Facon T
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - England
TA  - Leukemia
JT  - Leukemia
JID - 8704895
RN  - 0 (Antigens, CD20)
RN  - 9007-49-2 (DNA)
SB  - IM
MH  - Aged
MH  - Antigens, CD20/analysis
MH  - B-Lymphocytes/*ultrastructure
MH  - *Chromosome Aberrations
MH  - DNA/analysis
MH  - Female
MH  - Humans
MH  - In Situ Hybridization, Fluorescence
MH  - Male
MH  - Middle Aged
MH  - Multiple Myeloma/*blood
MH  - Plasma Cells/*ultrastructure
EDAT- 1997/07/01 00:00
MHDA- 1997/07/01 00:01
CRDT- 1997/07/01 00:00
PHST- 1997/07/01 00:00 [pubmed]
PHST- 1997/07/01 00:01 [medline]
PHST- 1997/07/01 00:00 [entrez]
PST - ppublish
SO  - Leukemia. 1997 Jul;11(7):1034-9.