PMID- 9205115
OWN - NLM
STAT- MEDLINE
DCOM- 19970902
LR  - 20061115
IS  - 0888-7543 (Print)
IS  - 0888-7543 (Linking)
VI  - 42
IP  - 3
DP  - 1997 Jun 15
TI  - A 2.8-Mb clone contig of the multiple endocrine neoplasia type 1 (MEN1) region at 
      11q13.
PG  - 436-45
AB  - Multiple endocrine neoplasia type 1 (MEN1) is an autosomal dominant disorder that 
      results in parathyroid, anterior pituitary, and pancreatic and duodenal endocrine 
      tumors in affected individuals. The MEN1 locus is tightly linked to the marker 
      PYGM on chromosome 11q13, and linkage analysis has placed the MEN1 gene within a 
      2-Mb interval flanked by D11S1883 and D11S449. As a step toward cloning the MEN1 
      gene, we have constructed a 2.8-Mb clone contig consisting of YAC and bacterial 
      clones (PAC, BAC, and P1) for the D11S480 to D11S913 region. The bacterial clones 
      alone represent nearly all of the 2.8-Mb contig. The contig was assembled based 
      on a high-density STS-content analysis of 79 genomic clones (YAC, PAC, BAC, and 
      P1) with 118 STSs. The STSs included 22 polymorphic markers and 20 transcripts, 
      with the remainder primarily derived from the end sequences of the genomic 
      clones. An independent cosmid contig for the 1-Mb PYGM-SEA region was also 
      generated. Support for correctness of the 2.8-Mb contig map comes from an 
      independent ordering of the clones by fiber-FISH. This sequence-ready contig will 
      be a useful resource for positional cloning of MEN1 and other disease genes whose 
      loci fall within this region.
FAU - Guru, S C
AU  - Guru SC
AD  - Laboratory of Gene Transfer, National Human Genome Research Institute, NIH, 
      Bethesda, Maryland 20892, USA.
FAU - Olufemi, S E
AU  - Olufemi SE
FAU - Manickam, P
AU  - Manickam P
FAU - Cummings, C
AU  - Cummings C
FAU - Gieser, L M
AU  - Gieser LM
FAU - Pike, B L
AU  - Pike BL
FAU - Bittner, M L
AU  - Bittner ML
FAU - Jiang, Y
AU  - Jiang Y
FAU - Chinault, A C
AU  - Chinault AC
FAU - Nowak, N J
AU  - Nowak NJ
FAU - Brzozowska, A
AU  - Brzozowska A
FAU - Crabtree, J S
AU  - Crabtree JS
FAU - Wang, Y
AU  - Wang Y
FAU - Roe, B A
AU  - Roe BA
FAU - Weisemann, J M
AU  - Weisemann JM
FAU - Boguski, M S
AU  - Boguski MS
FAU - Agarwal, S K
AU  - Agarwal SK
FAU - Burns, A L
AU  - Burns AL
FAU - Spiegel, A M
AU  - Spiegel AM
FAU - Marx, S J
AU  - Marx SJ
FAU - Flejter, W L
AU  - Flejter WL
FAU - de Jong, P J
AU  - de Jong PJ
FAU - Collins, F S
AU  - Collins FS
FAU - Chandrasekharappa, S C
AU  - Chandrasekharappa SC
LA  - eng
SI  - GENBANK/G33073
SI  - GENBANK/G33074
SI  - GENBANK/G33075
SI  - GENBANK/G33076
SI  - GENBANK/G33077
SI  - GENBANK/G33078
SI  - GENBANK/G33079
SI  - GENBANK/G33080
SI  - GENBANK/G33081
SI  - GENBANK/G33082
SI  - GENBANK/G33083
SI  - GENBANK/G33084
SI  - GENBANK/G33085
SI  - GENBANK/G33086
SI  - GENBANK/G33087
SI  - GENBANK/G33088
SI  - GENBANK/G33089
SI  - GENBANK/G33090
SI  - GENBANK/G33091
SI  - GENBANK/G33092
SI  - GENBANK/G33093
SI  - GENBANK/G33094
SI  - GENBANK/G33095
SI  - GENBANK/G33096
SI  - GENBANK/G33097
SI  - GENBANK/G33098
SI  - GENBANK/G33099
SI  - GENBANK/G33100
SI  - GENBANK/G33101
SI  - GENBANK/G33102
PT  - Journal Article
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - United States
TA  - Genomics
JT  - Genomics
JID - 8800135
RN  - 0 (MEN1 protein, human)
RN  - 0 (Neoplasm Proteins)
RN  - 0 (Proto-Oncogene Proteins)
SB  - IM
MH  - Chromosomes, Artificial, Yeast
MH  - *Chromosomes, Human, Pair 11
MH  - Cloning, Molecular
MH  - Cosmids
MH  - Humans
MH  - Hybrid Cells
MH  - In Situ Hybridization, Fluorescence
MH  - Multiple Endocrine Neoplasia/*genetics
MH  - Neoplasm Proteins/*genetics
MH  - *Proto-Oncogene Proteins
MH  - Sequence Tagged Sites
EDAT- 1997/06/15 00:00
MHDA- 1997/06/15 00:01
CRDT- 1997/06/15 00:00
PHST- 1997/06/15 00:00 [pubmed]
PHST- 1997/06/15 00:01 [medline]
PHST- 1997/06/15 00:00 [entrez]
AID - S0888-7543(97)94783-2 [pii]
AID - 10.1006/geno.1997.4783 [doi]
PST - ppublish
SO  - Genomics. 1997 Jun 15;42(3):436-45. doi: 10.1006/geno.1997.4783.