PMID- 9206101
OWN - NLM
STAT- MEDLINE
DCOM- 19970730
LR  - 20041117
IS  - 0366-6999 (Print)
IS  - 0366-6999 (Linking)
VI  - 109
IP  - 7
DP  - 1996 Jul
TI  - Spontaneous apoptosis in human colon tumor cell lines and the relation of wt p53 
      to apoptosis.
PG  - 537-41
AB  - OBJECTIVE: To examine spontaneous apoptosis of cultured human colon tumor cell 
      lines in vitro and to investigate the role of wild type (wt) p53 in regulation of 
      apoptosis induced by DNA-damaging treatment. METHODS: A model system of human 
      tumor progression involving three cell lines was used in this study for 
      examination of apoptosis. They were originally established from human colon 
      villous adenoma, including an early passage of non-tumorigenic cell line, V235E; 
      a late passage of weakly tumorigenic cell line, V235L; and a spontaneous 
      progressing highly tumorigenic cell line. V411. All of them maintain wt p53 
      expression. For identification of apoptosis, two tests were performed: 1. 
      morphology study using acridine orange (AO)/ethidium bromide (EB) stainning by 
      fluorescence microscopy; 2. DNA electrophoresis on agarose gel. P53 and WAF-1 (a 
      downstream gene of p53) expressions were analysed at mRNA level using Northern 
      blot technique. Apoptotic index of cell lines examined was measured by DNA 
      fluorescence assay. RESULTS: Spontaneous apoptosis was demonstrated in cell lines 
      of all stages of progression by both morphology and DNA agarose gel 
      electrophoresis. Apoptosis was further induced in V411 after treatment of cells 
      with 137Cs gamma-irradiation and accompanied by increases in p53 and WAF-1 
      expression. In contrast, a mutant p53 bearing human colon cancer cell line, 
      sw480, lacked spontaneous apoptosis, and upon irradiation neither induction of 
      apoptosis nor increase expression of p53 and WAF-1 were seen. CONCLUSIONS: 
      Apoptosis can be maintained in some human tumor cell lines despite transformation 
      and carcinogenesis. Wt p53 and WAF-1 products are two of the potential mediators 
      which effect apoptosis. Additionally, since apoptosis was enhanced by irradiation 
      in V411, but not in sw480, it suggests that wt p53 cancer cells are more 
      sensitive to DNA-damaging treatment than mutant p53 cancer cells. These finding 
      may have implications for cancer therapy.
FAU - Wang, C
AU  - Wang C
AD  - Internal Medicine Laboratory, First Affiliated Hospital of Nanjing Medical 
      University.
FAU - Eshleman, J
AU  - Eshleman J
FAU - Lutterbaugh, J
AU  - Lutterbaugh J
FAU - Bin, Y
AU  - Bin Y
FAU - Willson, J
AU  - Willson J
FAU - Markowitz, S
AU  - Markowitz S
LA  - eng
PT  - Journal Article
PL  - China
TA  - Chin Med J (Engl)
JT  - Chinese medical journal
JID - 7513795
SB  - IM
MH  - Adenoma, Villous/genetics/pathology
MH  - Apoptosis/*genetics
MH  - Colonic Neoplasms/genetics/*pathology
MH  - Gene Expression
MH  - Genes, p53
MH  - Humans
MH  - Tumor Cells, Cultured
EDAT- 1996/07/01 00:00
MHDA- 1996/07/01 00:01
CRDT- 1996/07/01 00:00
PHST- 1996/07/01 00:00 [pubmed]
PHST- 1996/07/01 00:01 [medline]
PHST- 1996/07/01 00:00 [entrez]
PST - ppublish
SO  - Chin Med J (Engl). 1996 Jul;109(7):537-41.