PMID- 9208445
OWN - NLM
STAT- MEDLINE
DCOM- 19970822
LR  - 20031114
IS  - 0928-4249 (Print)
IS  - 0928-4249 (Linking)
VI  - 28
IP  - 3
DP  - 1997 May-Jun
TI  - Antigenic variability of porcine reproductive and respiratory syndrome (PRRS) 
      virus isolates. Influence of virus passage in pig.
PG  - 247-57
AB  - In order to study the antigenic variability of porcine reproductive and 
      respiratory syndrome virus (PRRSV), 18 European and Canadian field isolates were 
      analysed with a panel of 15 monoclonal antibodies (MAbs) raised against five 
      different European and one Canadian PRRSV isolates. The antigenic pattern of 
      these isolates was used to infer their phylogenetic relationships. Isolates which 
      had the same reactivity pattern were gathered together so that five antigenic 
      profiles were analysed. The pairwise distances between these groups were defined 
      based on antigenic pattern differences. Two main antigenic groups were obtained, 
      discriminating between the European and the Canadian populations, as illustrated 
      by the great distance observed between European and Canadian isolates (D = 0.5619 
      +/- 0.0625) compared to the distance between European isolates (D = 0.1524 +/- 
      0.0735). The distance matrix allowed also the construction of a tree diagram. 
      Bootstrap analysis was performed to test the confidence in the branching. The 
      tree diagram confirmed the distinction between the European and the Canadian 
      PRRSV populations. Antigenic variability between an isolate and its progeny 
      recovered after one or two passages in vivo was examined on six isolates. It was 
      restricted to the GP3 protein of the virus.
FAU - Le Gall, A
AU  - Le Gall A
AD  - UR virologie immunologie porcine, Cneva, Ploufragan, France.
FAU - Albina, E
AU  - Albina E
FAU - Magar, R
AU  - Magar R
FAU - Gauthier, J P
AU  - Gauthier JP
LA  - eng
PT  - Journal Article
PL  - England
TA  - Vet Res
JT  - Veterinary research
JID - 9309551
RN  - 0 (Antibodies, Monoclonal)
RN  - 0 (Antigens, Viral)
RN  - 0 (Epitopes)
SB  - IM
MH  - Animals
MH  - Antibodies, Monoclonal
MH  - Antigen-Antibody Reactions
MH  - *Antigenic Variation
MH  - Antigens, Viral/analysis/*genetics
MH  - Canada
MH  - Epitopes/analysis
MH  - Europe
MH  - Phylogeny
MH  - Porcine Reproductive and Respiratory Syndrome/*virology
MH  - Porcine respiratory and reproductive syndrome 
      virus/*genetics/*immunology/isolation & purification
MH  - Swine
EDAT- 1997/05/01 00:00
MHDA- 1997/05/01 00:01
CRDT- 1997/05/01 00:00
PHST- 1997/05/01 00:00 [pubmed]
PHST- 1997/05/01 00:01 [medline]
PHST- 1997/05/01 00:00 [entrez]
AID - S0928424997820081 [pii]
PST - ppublish
SO  - Vet Res. 1997 May-Jun;28(3):247-57.