PMID- 9209467
OWN - NLM
STAT- MEDLINE
DCOM- 19970717
LR  - 20190816
IS  - 0165-4608 (Print)
IS  - 0165-4608 (Linking)
VI  - 96
IP  - 1
DP  - 1997 Jul 1
TI  - Cytogenetic and molecular genetic characterization of a chromosome 2 
      rearrangement in a case of human papillary thyroid carcinoma with radiation 
      history.
PG  - 30-6
AB  - Karyotype analysis of a primary culture from a case of papillary thyroid cancer 
      (PTC) showed an abnormal short arm of one homologue of chromosome 2 as sole 
      abnormality in 4 of 16 metaphases. Based on G-banding analysis, two different 
      aberration types on chromosome 2 could be assumed representing either a 
      del(2)(p22-23) or a pericentric inversion. Further comparative genomic 
      hybridization (CGH) analysis as well as fluorescence in situ hybridization (FISH) 
      analysis were performed to confirm the assumed alterations. While CGH analysis 
      showed no loss of chromosome 2 material, FISH with yeast artificial chromosome 
      (YAC) probes homologous to the region 2p22-23 demonstrated two pericentric 
      inversions of chromosome 2 involving different breakpoints on 2p in 6.8% and 4.2% 
      of the metaphases, respectively. Polymerase chain reaction (PCR) analysis with 
      degenerated oligonucleotide primers that bind within the conserved catalytic 
      domain of tyrosine kinase (tk) genes resulted in amplification products with DNA 
      of YAC 851D11 suggesting the presence of such genes at or near the translocation 
      breakpoint.
FAU - Lehmann, L
AU  - Lehmann L
AD  - Institute of Radiobiology, GSF-National Research Center for Environment and 
      Health, Munich, Germany.
FAU - Greulich, K M
AU  - Greulich KM
FAU - Zitzelsberger, H
AU  - Zitzelsberger H
FAU - Negele, T
AU  - Negele T
FAU - Spelsberg, F
AU  - Spelsberg F
FAU - Bauchinger, M
AU  - Bauchinger M
FAU - Weier, H U
AU  - Weier HU
LA  - eng
PT  - Case Reports
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PT  - Research Support, U.S. Gov't, Non-P.H.S.
PL  - United States
TA  - Cancer Genet Cytogenet
JT  - Cancer genetics and cytogenetics
JID - 7909240
SB  - IM
MH  - Adult
MH  - Carcinoma, Papillary/*genetics
MH  - *Chromosome Inversion
MH  - Chromosomes, Artificial, Yeast/genetics
MH  - Chromosomes, Human, Pair 2/*genetics
MH  - Humans
MH  - In Situ Hybridization, Fluorescence
MH  - Male
MH  - Polymerase Chain Reaction
MH  - Thyroid Neoplasms/*genetics
EDAT- 1997/07/01 00:00
MHDA- 1997/07/01 00:01
CRDT- 1997/07/01 00:00
PHST- 1997/07/01 00:00 [pubmed]
PHST- 1997/07/01 00:01 [medline]
PHST- 1997/07/01 00:00 [entrez]
AID - S0165460896002798 [pii]
AID - 10.1016/s0165-4608(96)00279-8 [doi]
PST - ppublish
SO  - Cancer Genet Cytogenet. 1997 Jul 1;96(1):30-6. doi: 
      10.1016/s0165-4608(96)00279-8.