PMID- 9209656
OWN - NLM
STAT- MEDLINE
DCOM- 19970905
LR  - 20161017
IS  - 0923-7534 (Print)
IS  - 0923-7534 (Linking)
VI  - 8 Suppl 2
DP  - 1997
TI  - Integration of Epstein-Barr virus in Burkitt's lymphoma cells leads to a region 
      of enhanced chromosome instability.
PG  - 131-5
AB  - BACKGROUND: Several lymphomas are associated with Epstein-Barr virus (EBV) 
      infection. However EBV is not detectable in 100% of cases using standard staining 
      techniques. It still remains an open question whether in these EBV-negative cases 
      EBV has never infected the cell, whether it has infected the cell and escapes 
      conventional screening methods, or whether it has been lost again after initial 
      infection. MATERIALS AND METHODS: The physical status of EBV in the Burkitt's 
      lymphoma cell line BL60-P7 as well as in three somatic cell hybrids between 
      BL60-P7 and its autologous EBV-immortalized lymphoblastoid cell line IARC 277 was 
      analyzed using conventional cytogenetics, Southern blotting, and fluorescence in 
      situ hybridization (FISH). RESULTS: Integration of EBV into the host genome of 
      the lymphoma cell line BL60-P7 leads to an achromatic gap which causes a 
      'vulnerable site'. In hybrid cells, loss of integrated EBV, together with an 
      adjacent chromosomal fragment, occurs during long-term cultivation. The 
      integrated EBV genome, including genes encoding for LMP and EBER, is partly 
      deleted. CONCLUSION: We assume that integration of EBV into the host cell genome 
      could be a more common event in lymphoma cells. Partially deleted EBV might 
      escape standard detection assays. The integration might constitute a chromosomal 
      region prone to break events akin to the phenomenon of fragile sites, leading to 
      the loss of viral DNA as well as chromosomal DNA. This observation makes it 
      tempting to speculate that under certain conditions EBV can act in 
      lymphomagenesis by a so-called hit-and-run mechanism.
FAU - Jox, A
AU  - Jox A
AD  - Department of Internal Medicine I, University of Cologne, Germany.
FAU - Rohen, C
AU  - Rohen C
FAU - Belge, G
AU  - Belge G
FAU - Bartnitzke, S
AU  - Bartnitzke S
FAU - Pawlita, M
AU  - Pawlita M
FAU - Diehl, V
AU  - Diehl V
FAU - Bullerdiek, J
AU  - Bullerdiek J
FAU - Wolf, J
AU  - Wolf J
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - England
TA  - Ann Oncol
JT  - Annals of oncology : official journal of the European Society for Medical 
      Oncology
JID - 9007735
RN  - 0 (Chromatin)
SB  - IM
MH  - Blotting, Southern
MH  - Burkitt Lymphoma/*virology
MH  - Chromatin/*genetics
MH  - *Chromosome Deletion
MH  - *Genome, Viral
MH  - Herpesvirus 4, Human/*isolation & purification
MH  - Humans
MH  - In Situ Hybridization, Fluorescence
MH  - Karyotyping
MH  - Tumor Cells, Cultured
EDAT- 1997/01/01 00:00
MHDA- 1997/01/01 00:01
CRDT- 1997/01/01 00:00
PHST- 1997/01/01 00:00 [pubmed]
PHST- 1997/01/01 00:01 [medline]
PHST- 1997/01/01 00:00 [entrez]
PST - ppublish
SO  - Ann Oncol. 1997;8 Suppl 2:131-5.