PMID- 9210176
OWN - NLM
STAT- MEDLINE
DCOM- 19970911
LR  - 20190818
IS  - 0196-9781 (Print)
IS  - 0196-9781 (Linking)
VI  - 18
IP  - 4
DP  - 1997
TI  - Differential responsiveness of proximal and distal colonic mucosa to gastrin.
PG  - 559-65
AB  - In vivo and in vitro experiments were performed to examine the responsiveness of 
      the proximal and distal colonic mucosa to the growth-promoting action of gastrin. 
      Infusion (osmotic minipump) of gastrin G-17-I (250 ng/kg/h) for 5 days to 
      4-month-old male Fischer-344 rats resulted in a significant (90-150%) increase in 
      proliferative activity (as assessed by BrdU or PCNA immunoreactivity) in the 
      distal colonic mucosa. In contrast, gastrin caused no apparent change in 
      proliferative activity in the proximal colon. Because tyrosine kinases (Tyr-ks) 
      are thought to be critically involved in regulating the trophic action of 
      gastrin, responsiveness of isolated colonocytes from both segments of the colon 
      to gastrin (1 x 10(-9) M) was also examined. Exposure of isolated colonocytes 
      from the distal, but not from the proximal, colon to gastrin for 2 min resulted 
      in a significant (73%) stimulation in Tyr-k activity. This was also accompanied 
      by a marked rise in phosphorylation of at least six membrane proteins with M, of 
      55, 60, 70, 94, and 170 kDa. Tyr-k activity induced by gastrin in colonocytes 
      from the distal colon was inhibited by tyrphostin (3.2 microM) but not by 
      staurosporine (20 nM). In colonocytes from the distal colon, gastrin also 
      stimulated phospholipase C (PLC) activity, which could also be inhibited by 
      tyrphostin, but not by staurosporine. We conclude that mucosa of the distal, but 
      not the proximal, colon responds to the trophic action of gastrin. Tyr-ks are 
      thought to be involved in the regulation of this process.
FAU - Malecka-Panas, E
AU  - Malecka-Panas E
AD  - VA Medical Center, Detroit, MI 48201, USA.
FAU - Fligiel, S E
AU  - Fligiel SE
FAU - Jaszewski, R
AU  - Jaszewski R
FAU - Majumdar, A P
AU  - Majumdar AP
LA  - eng
GR  - AG 08438/AG/NIA NIH HHS/United States
PT  - Comparative Study
PT  - Journal Article
PT  - Research Support, U.S. Gov't, Non-P.H.S.
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - United States
TA  - Peptides
JT  - Peptides
JID - 8008690
RN  - 0 (Gastrins)
SB  - IM
MH  - Animals
MH  - Colon/cytology/*drug effects
MH  - Drug Evaluation, Preclinical
MH  - Gastrins/*pharmacology
MH  - Infusion Pumps, Implantable
MH  - Intestinal Mucosa/cytology/*drug effects
MH  - Male
MH  - Phosphorylation
MH  - Rats
MH  - Rats, Inbred F344
EDAT- 1997/01/01 00:00
MHDA- 1997/01/01 00:01
CRDT- 1997/01/01 00:00
PHST- 1997/01/01 00:00 [pubmed]
PHST- 1997/01/01 00:01 [medline]
PHST- 1997/01/01 00:00 [entrez]
AID - S0196-9781(97)00061-2 [pii]
AID - 10.1016/s0196-9781(97)00061-2 [doi]
PST - ppublish
SO  - Peptides. 1997;18(4):559-65. doi: 10.1016/s0196-9781(97)00061-2.