PMID- 9210282
OWN - NLM
STAT- MEDLINE
DCOM- 19970814
LR  - 20061115
IS  - 0882-8245 (Print)
IS  - 0882-8245 (Linking)
VI  - 10
IP  - 2
DP  - 1997
TI  - Cell-mediated immunity to porcine reproductive and respiratory syndrome virus in 
      swine.
PG  - 83-94
AB  - Cell-mediated immunity has been demonstrated to be a necessary component of 
      immunity against viral infection. Methods to detect T-cell mediated immune 
      responses to porcine reproductive and respiratory syndrome virus (PRRSV) 
      infection were established both in vitro as lymphocyte proliferation and in vivo 
      as delayed-type hypersensitivity response (DTH). Optimal conditions for detection 
      of lymphocyte proliferation were determined by testing different antigen 
      concentrations and various stimulation periods. The proliferation response to 
      PRRSV was antigen-specific and dose-dependent. The kinetics of the T-cell 
      proliferation response to PRRSV were analyzed after primary and secondary 
      exposure to virus. Lymphocyte proliferation was first detected at four weeks 
      post-infection (PI), peaked at 7 weeks PI, and declined after 11 weeks PI. The 
      secondary response increased in magnitude. Experiments with blocking antibodies 
      to porcine leukocyte antigens demonstrated that CD4+ T-cells were the major 
      effector cells in the proliferation response. The in vivo response to PRRSV was 
      shown by detection of a dose-dependent DTH reaction in infected pigs after 
      intradermal challenge with UV-inactivated virus. These results demonstrate that 
      pigs generate specific T-cell responses on PRRSV infection and provide a 
      foundation for studying their role in protection.
FAU - Bautista, E M
AU  - Bautista EM
AD  - Department of Clinical and Population Sciences, College of Veterinary Medicine, 
      University of Minnesota, St. Paul, USA.
FAU - Molitor, T W
AU  - Molitor TW
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - Viral Immunol
JT  - Viral immunology
JID - 8801552
SB  - IM
MH  - Animals
MH  - CD4-Positive T-Lymphocytes/immunology/virology
MH  - CD8-Positive T-Lymphocytes/immunology/virology
MH  - Hypersensitivity, Delayed/immunology/veterinary/virology
MH  - Immunization
MH  - Immunization, Secondary
MH  - *Lymphocyte Activation
MH  - Porcine Reproductive and Respiratory Syndrome/*immunology
MH  - Porcine respiratory and reproductive syndrome virus/*immunology
MH  - Swine
MH  - T-Lymphocytes/immunology/virology
EDAT- 1997/01/01 00:00
MHDA- 1997/01/01 00:01
CRDT- 1997/01/01 00:00
PHST- 1997/01/01 00:00 [pubmed]
PHST- 1997/01/01 00:01 [medline]
PHST- 1997/01/01 00:00 [entrez]
AID - 10.1089/vim.1997.10.83 [doi]
PST - ppublish
SO  - Viral Immunol. 1997;10(2):83-94. doi: 10.1089/vim.1997.10.83.