PMID- 9210529
OWN - NLM
STAT- MEDLINE
DCOM- 19970826
LR  - 20061115
IS  - 0360-4012 (Print)
IS  - 0360-4012 (Linking)
VI  - 48
IP  - 6
DP  - 1997 Jun 15
TI  - Platelet-derived growth factor delays oligodendrocyte differentiation and axonal 
      myelination in vivo in the anterior medullary velum of the developing rat.
PG  - 588-96
AB  - The AA dimeric form of platelet-derived growth factor (PDGF-AA) is implicated in 
      the differentiation of cells of the oligodendrocyte lineage, which express PDGF 
      receptors of the alpha subunit type (PDGF-alphaR). In the present study, we show 
      that a single injection of PDGF-AA into the cerebrospinal fluid of neonatal rats 
      delays oligodendrocyte differentiation and interrupts the progress of myelination 
      in the anterior medullary velum (AMV), a white matter tract roofing the IVth 
      ventricle of the brain. PDGF-AA or saline was injected intrathecally in postnatal 
      day (P) 7 rats, and the AMV was subsequently removed and immunolabelled with the 
      oligodendrocyte-specific antibody Rip, at P9, P12, and P21, corresponding to 
      postinjection days (PID) 2, 5, and 14. At P9 (PID2), myelination was retarded in 
      PDGF-AA-treated rats as opposed to saline-treated controls but progressed rapidly 
      after P12 (PID5). Quantification supported the qualitative observations that 
      PDGF-AA mediated an acute decrease in the number of Rip+ oligodendrocytes at 
      P9-12, which largely recovered by P21, suggesting that PDGF-AA may have delayed 
      recruitment of myelinating oligodendrocytes. However, the definitive number of 
      Rip+ oligodendrocytes in the AMV was not increased, suggesting that its action as 
      a promoter of early oligodendrocyte survival may not ultimately affect the 
      definitive number of myelinating oliogdendrocytes in vivo. We discuss the 
      possibilities that excess PDGF-AA may have acted on early oligodendrocytes 
      (precursors or preoligodendrocytes) to either (1) delay their differentiation by 
      maintaining them in the cell cycle or (2) accelerate their differentiation, which 
      may result in premature cell death in the absence of synchronised survival 
      signals. This study supports a role for PDGF-AA in the timing of oligodendrocyte 
      differentiation in vivo, as has been shown in vitro.
FAU - Butt, A M
AU  - Butt AM
AD  - Division of Physiology, UMDS, Guy's and St. Thomas' Hospitals, London, United 
      Kingdom. a.butt@umds.ac.uk
FAU - Hornby, M F
AU  - Hornby MF
FAU - Kirvell, S
AU  - Kirvell S
FAU - Berry, M
AU  - Berry M
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - J Neurosci Res
JT  - Journal of neuroscience research
JID - 7600111
RN  - 0 (Platelet-Derived Growth Factor)
SB  - IM
MH  - Animals
MH  - Axons/*drug effects/metabolism
MH  - Cell Count
MH  - Cell Differentiation/drug effects
MH  - Cell Survival
MH  - Cerebral Ventricles/cytology/*drug effects/growth & development
MH  - Depression, Chemical
MH  - Injections, Spinal
MH  - Myelin Sheath/*drug effects/physiology
MH  - Oligodendroglia/cytology/*drug effects
MH  - Platelet-Derived Growth Factor/administration & dosage/*pharmacology/toxicity
MH  - Rats
MH  - Rats, Wistar
MH  - Stem Cells/cytology/drug effects
EDAT- 1997/06/15 00:00
MHDA- 2000/06/20 09:00
CRDT- 1997/06/15 00:00
PHST- 1997/06/15 00:00 [pubmed]
PHST- 2000/06/20 09:00 [medline]
PHST- 1997/06/15 00:00 [entrez]
AID - 10.1002/(SICI)1097-4547(19970615)48:6<588::AID-JNR12>3.0.CO;2-R [pii]
PST - ppublish
SO  - J Neurosci Res. 1997 Jun 15;48(6):588-96.