PMID- 9211354
OWN - NLM
STAT- MEDLINE
DCOM- 19970930
LR  - 20190725
IS  - 0085-2538 (Print)
IS  - 0085-2538 (Linking)
VI  - 52
IP  - 1
DP  - 1997 Jul
TI  - Identification of promoter activity and differential expression of transcripts 
      encoding the murine stromelysin-1 gene in renal cells.
PG  - 120-9
AB  - Stromelysin-1, matrix metalloproteinase-3 (MMP-3), is an important endopeptidase 
      selectively expressed by somatic cells in organ tissues. The renal 
      tubulointerstitium, for example, comprises tubular epithelium and interstitial 
      fibroblasts forming the principal mass of the kidney. We observed that mRNA 
      encoding stromelysin-1 is detectable in murine renal fibroblasts, but not in 
      proximal tubular epithelium. Transcripts measured by RNase protection assay in 
      renal fibroblasts increase following exposure to phorbol ester, and thereafter, 
      activated stromelysin-1 protein can be detected in culture media by Western 
      blotting. A 6.4 Kb genomic clone containing the putative stromelysin-1 promoter 
      was isolated and a relevant 2.1 Kb PstI restriction fragment including 2.1 Kb of 
      the immediate 5'-flanking region was sequenced on both strands. Two 
      transcriptional start sites were identified by primer extension; the major start 
      site corresponded to a previously established position in the rat promoter, and a 
      second undescribed minor transcriptional start site was located 16 bp upstream of 
      the primary site. A HiNF-A chromatin-activating element at -106 bp was found in 
      the early promoter region of pR336 and an active AP-1 site at -72 bp with an 
      Ets/PEA-3 motif at -203 bp was suggested by transient transfection of luciferase 
      minigenes into renal fibroblasts responsive to phorbol ester. This Ets element 
      was identical to a site in the early promoter of the fibroblast-specific gene 
      FSP1. A baseline enhancement in activity of pR336 in fibroblasts was further 
      observed with the addition of 5' flanking sequence out to -1980 bp. This 
      additional region of flanking sequence contains two modular regions: one of 
      multiple PEA-3 elements between -684 bp and -1955 bp and a second region between 
      -1929 bp and -1980 bps containing a second AP-1 site at -1929 bp, a MBF-1/ MEP-1 
      metal binding site, and a PPAR peroxisome proliferator element at -1950 bp. Our 
      findings implicate a gene structure with expected activity in a mesenchymal 
      phenotype. The PKC-dependent regulation of the stromelysin-1 gene supports the 
      notion that it may be modulated during inflammation or tissue remodeling.
FAU - Yee, J
AU  - Yee J
AD  - Department of Medicine, University of Texas Health Science Center, San Antonio, 
      USA.
FAU - Kuncio, G S
AU  - Kuncio GS
FAU - Bhandari, B
AU  - Bhandari B
FAU - Shihab, F S
AU  - Shihab FS
FAU - Neilson, E G
AU  - Neilson EG
LA  - eng
GR  - DK-07006/DK/NIDDK NIH HHS/United States
GR  - DK-30280/DK/NIDDK NIH HHS/United States
GR  - DK-45191/DK/NIDDK NIH HHS/United States
GR  - etc.
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - United States
TA  - Kidney Int
JT  - Kidney international
JID - 0323470
RN  - 0 (Culture Media, Conditioned)
RN  - 0 (RNA, Messenger)
RN  - EC 3.4.24.17 (Matrix Metalloproteinase 3)
SB  - IM
MH  - 3T3 Cells
MH  - Animals
MH  - Base Sequence
MH  - Blotting, Northern
MH  - Blotting, Southern
MH  - Cells, Cultured
MH  - Culture Media, Conditioned/chemistry
MH  - Gene Expression
MH  - Kidney/*metabolism
MH  - Matrix Metalloproteinase 3/biosynthesis/*genetics
MH  - Mice
MH  - Molecular Sequence Data
MH  - Promoter Regions, Genetic/*genetics
MH  - RNA, Messenger/analysis
MH  - Rats
MH  - Sequence Homology, Nucleic Acid
MH  - Transfection
EDAT- 1997/07/01 00:00
MHDA- 1997/07/01 00:01
CRDT- 1997/07/01 00:00
PHST- 1997/07/01 00:00 [pubmed]
PHST- 1997/07/01 00:01 [medline]
PHST- 1997/07/01 00:00 [entrez]
AID - S0085-2538(15)60151-X [pii]
AID - 10.1038/ki.1997.311 [doi]
PST - ppublish
SO  - Kidney Int. 1997 Jul;52(1):120-9. doi: 10.1038/ki.1997.311.