PMID- 9212161
OWN - NLM
STAT- MEDLINE
DCOM- 19970725
LR  - 20111117
IS  - 1044-5498 (Print)
IS  - 1044-5498 (Linking)
VI  - 16
IP  - 6
DP  - 1997 Jun
TI  - Cloning, expression, and chromosomal localization to 11p12-13 of a human 
      LIM/HOMEOBOX gene, hLim-1.
PG  - 671-8
AB  - We have identified a putative transcription factor, designated hLim-1, from human 
      fetal brain using degenerate polymerase chain reaction (PCR) and cDNA library 
      screening. The deduced open reading frame, derived from sequencing a 3.0-kb 
      hLim-1 cDNA, encodes a protein of 384 amino acids with two cysteine-rich LIM 
      domains and one homeobox (HOX) DNA-binding domain. The nucleotide sequence of 
      hLim-1 cDNA is 87% identical to mouse Lim-1 and the predicted amino acid sequence 
      is greater than 97% conserved. Expression patterns of hLim-1 were evaluated by 
      Northern analysis and reverse transcription (RT)-PCR coupled with Southern 
      blotting. HLim-1 expression was observed in human brain, thymus, and tonsillar 
      tissue. Expression of hLim-1 was also observed in 58% of acute myelogenous 
      leukemia (AML) cell lines and in four of five primary samples from patients with 
      chronic myeloid leukemia (CML) in myeloid blast transformation. The gene encoding 
      hLim-1 was mapped using fluorescence in situ hybridization (FISH) to human 
      chromosome 11p12-13. The expression pattern and structural characteristics of the 
      hLim-1 gene suggest that it encodes a transcriptional regulatory protein involved 
      in the control of differentiation and development of neural and lymphoid cells. 
      Its expression in CML in blast crisis suggests that it may be involved with 
      progression in this disease; a prospective study is required to confirm this.
FAU - Dong, W F
AU  - Dong WF
AD  - Ontario Cancer Institute, Princess Margaret Hospital, Department of Medicine, 
      University of Toronto, Canada.
FAU - Heng, H H
AU  - Heng HH
FAU - Lowsky, R
AU  - Lowsky R
FAU - Xu, Y
AU  - Xu Y
FAU - DeCoteau, J F
AU  - DeCoteau JF
FAU - Shi, X M
AU  - Shi XM
FAU - Tsui, L C
AU  - Tsui LC
FAU - Minden, M D
AU  - Minden MD
LA  - eng
SI  - GENBANK/U14755
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - DNA Cell Biol
JT  - DNA and cell biology
JID - 9004522
RN  - 0 (DNA, Complementary)
RN  - 0 (Homeodomain Proteins)
RN  - 0 (LHX1 protein, human)
RN  - 0 (LIM-Homeodomain Proteins)
RN  - 0 (Lhx1 protein, mouse)
RN  - 0 (Transcription Factors)
SB  - IM
MH  - Amino Acid Sequence
MH  - Animals
MH  - Base Sequence
MH  - Chromosome Mapping
MH  - *Chromosomes, Human, Pair 11
MH  - Cloning, Molecular
MH  - DNA, Complementary
MH  - Homeodomain Proteins/*genetics
MH  - Humans
MH  - In Situ Hybridization, Fluorescence
MH  - LIM-Homeodomain Proteins
MH  - Mice
MH  - Molecular Sequence Data
MH  - Sequence Homology, Amino Acid
MH  - Transcription Factors
EDAT- 1997/06/01 00:00
MHDA- 1997/06/01 00:01
CRDT- 1997/06/01 00:00
PHST- 1997/06/01 00:00 [pubmed]
PHST- 1997/06/01 00:01 [medline]
PHST- 1997/06/01 00:00 [entrez]
AID - 10.1089/dna.1997.16.671 [doi]
PST - ppublish
SO  - DNA Cell Biol. 1997 Jun;16(6):671-8. doi: 10.1089/dna.1997.16.671.