PMID- 9212219
OWN - NLM
STAT- MEDLINE
DCOM- 19970723
LR  - 20190708
IS  - 0020-7136 (Print)
IS  - 0020-7136 (Linking)
VI  - 72
IP  - 1
DP  - 1997 Jul 3
TI  - Detection of 14q32.33 translocation and t(11;14) in interphase nuclei of chronic 
      B-cell leukemia/lymphomas by in situ hybridization.
PG  - 31-8
AB  - Abnormalities of chromosome 14 involving band q32.33 are among the most commonly 
      observed cytogenetic alterations in B-cell malignancies. To assess the incidence 
      and pathogenetic implications of 14q32.33 translocation in chronic B-cell 
      leukemia/lymphomas, we performed fluorescence in situ hybridization (FISH) 
      analysis with variable region (V(H)) and gamma constant region (Cgamma) gene 
      probes in 37 patients with these disorders. Chromosome 14q32.33 translocation was 
      detected in 2 of 18 patients with chronic lymphocytic leukemia (CLL), 1 of 2 with 
      CLL of mixed cell types (CLL/PL), 1 of 2 with pro-lymphocytic leukemia (PLL), 5 
      of 6 with leukemic mantle-cell lymphoma (MCL), 2 of 7 with splenic B-cell 
      leukemia/lymphoma of possible marginal zone origin (SBLL) and 2 with leukemic 
      follicular lymphoma (FL). To further characterize 14q32.33 translocations in 
      these patients, we developed a new procedure using double-color FISH with PRAD1, 
      BCL2, V(H) and Cgamma gene probes. Chromosome t(11;14) was detected in 1 patient 
      with CLL/PL, 1 with PLL and 5 with MCL. Chromosome t(14;18) was detected in 2 
      patients with FL. In a PLL patient with t(11;14), the cosmid CPP29 containing the 
      PRAD1 gene and its 5'-flanking region split and co-localized with both Cgamma and 
      V(H) gene probes, thus spanning the breakpoint. In CLL and SBLL patients, donor 
      chromosomes were other than chromosomes 2, 11, 18 and 19, suggesting the 
      involvement of a novel oncogene(s) in the pathogenesis of these diseases. 
      Interphase FISH rapidly detected 14q32.33 translocation, t(11;14) and t(14;18) in 
      B-cell malignancies with low mitotic activity at the single-cell level, 
      facilitating the correlation of the molecular features of these translocations 
      with clinical characteristics.
FAU - Takashima, T
AU  - Takashima T
AD  - Third Department of Internal Medicine, Kyoto Prefectural University of Medicine, 
      Japan.
FAU - Itoh, M
AU  - Itoh M
FAU - Ueda, Y
AU  - Ueda Y
FAU - Nishida, K
AU  - Nishida K
FAU - Tamaki, T
AU  - Tamaki T
FAU - Misawa, S
AU  - Misawa S
FAU - Abe, T
AU  - Abe T
FAU - Seto, M
AU  - Seto M
FAU - Machii, T
AU  - Machii T
FAU - Taniwaki, M
AU  - Taniwaki M
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - Int J Cancer
JT  - International journal of cancer
JID - 0042124
SB  - IM
MH  - Adolescent
MH  - Adult
MH  - Aged
MH  - Aged, 80 and over
MH  - Cell Nucleus/ultrastructure
MH  - *Chromosomes, Human, Pair 11
MH  - *Chromosomes, Human, Pair 14
MH  - Chronic Disease
MH  - Female
MH  - Humans
MH  - In Situ Hybridization, Fluorescence
MH  - Interphase
MH  - Leukemia, B-Cell/*genetics
MH  - Lymphoma/*genetics
MH  - Male
MH  - Middle Aged
MH  - *Translocation, Genetic
EDAT- 1997/07/03 00:00
MHDA- 2000/06/20 09:00
CRDT- 1997/07/03 00:00
PHST- 1997/07/03 00:00 [pubmed]
PHST- 2000/06/20 09:00 [medline]
PHST- 1997/07/03 00:00 [entrez]
AID - 10.1002/(SICI)1097-0215(19970703)72:1<31::AID-IJC4>3.0.CO;2-5 [pii]
AID - 10.1002/(sici)1097-0215(19970703)72:1<31::aid-ijc4>3.0.co;2-5 [doi]
PST - ppublish
SO  - Int J Cancer. 1997 Jul 3;72(1):31-8. doi: 
      10.1002/(sici)1097-0215(19970703)72:1<31::aid-ijc4>3.0.co;2-5.