PMID- 9218606
OWN - NLM
STAT- MEDLINE
DCOM- 19970805
LR  - 20031114
IS  - 0022-1767 (Print)
IS  - 0022-1767 (Linking)
VI  - 159
IP  - 2
DP  - 1997 Jul 15
TI  - Chemokine expression in experimental tubulointerstitial nephritis.
PG  - 870-6
AB  - Chemokines may be important in the pathogenesis of leukocyte infiltration in 
      tubulointerstitial nephritis associated with glomerular disease. We studied the 
      renal cortical expression of the C-C (macrophage inflammatory protein-1alpha 
      (MIP-1alpha)), monocyte chemotactic protein-1 (MCP-1), and RANTES) and C-X-C 
      (interferon-inducible protein-10 (IP-10), MIP-2, and cytokine-induced neutrophil 
      chemoattractant (CINC)) chemokines 4, 6, 8, 10, 14, and 21 days after the 
      induction of puromycin aminonucleoside (PAN) nephrosis. There was a 7- to 10-fold 
      increase in the steady state mRNA expression of IP-10 and MCP-1 in the renal 
      cortex of rats 6 to 8 days after the administration of PAN that declines 
      thereafter reaching control values by day 21. The site of IP-10 and MCP-1 mRNA 
      production was localized to intrinsic tubulointerstitial cells and not to 
      infiltrating monocytes or macrophages. By comparison, there was a low basal 
      expression of RANTES mRNA in the renal cortex of nephrotic rats that did not 
      differ from those of control rats. In contrast, CINC, MIP-2, and MIP-1alpha mRNAs 
      were not detected. Translation of MCP-1 mRNA into protein was confirmed with an 
      ELISA. These changes in chemokine gene expression were associated with a 
      tubulointerstitial T lymphocyte and macrophage infiltration beginning on day 6 
      that peaked on day 10. Administration of a neutralizing Ab to rat MCP-1 (n = 5) 
      beginning on day 4 resulted in a 45% decline in tubulointerstitial macrophage 
      infiltration from 8.4 +/- 1.3% to 4.6 +/- 0.4% (p < 0.001) on day 6. These data 
      provide evidence that MCP-1, and possibly IP-10, are important in the 
      pathogenesis of monocyte/macrophage infiltration in the tubulointerstitial 
      nephritis associated with PAN nephrosis.
FAU - Tang, W W
AU  - Tang WW
AD  - Department of Pathology, Amgen Inc., Thousand Oaks, CA 91320, USA.
FAU - Qi, M
AU  - Qi M
FAU - Warren, J S
AU  - Warren JS
FAU - Van, G Y
AU  - Van GY
LA  - eng
PT  - Journal Article
PL  - United States
TA  - J Immunol
JT  - Journal of immunology (Baltimore, Md. : 1950)
JID - 2985117R
RN  - 0 (Chemokines)
RN  - 0 (RNA, Messenger)
RN  - 58-60-6 (Puromycin Aminonucleoside)
SB  - IM
MH  - Animals
MH  - Chemokines/*biosynthesis
MH  - Immunohistochemistry
MH  - Kidney Cortex/*immunology/pathology
MH  - Nephritis, Interstitial/chemically induced/*immunology
MH  - Puromycin Aminonucleoside
MH  - RNA, Messenger/analysis
MH  - Rats
MH  - Rats, Inbred Lew
EDAT- 1997/07/15 00:00
MHDA- 1997/07/15 00:01
CRDT- 1997/07/15 00:00
PHST- 1997/07/15 00:00 [pubmed]
PHST- 1997/07/15 00:01 [medline]
PHST- 1997/07/15 00:00 [entrez]
PST - ppublish
SO  - J Immunol. 1997 Jul 15;159(2):870-6.