PMID- 9218997
OWN - NLM
STAT- MEDLINE
DCOM- 19970826
LR  - 20191024
IS  - 1045-2257 (Print)
IS  - 1045-2257 (Linking)
VI  - 19
IP  - 3
DP  - 1997 Jul
TI  - Frequent gain of copy number on the long arm of chromosome 20 in human pancreatic 
      adenocarcinoma.
PG  - 161-9
AB  - We have used comparative genomic hybridization (CGH) to survey genomic regions 
      with aberrant copy numbers of DNA sequences in pancreatic adenocarcinoma. In 12 
      cell lines and 6 primary tumors from 18 patients with pancreatic adenocarcinomas, 
      highly frequent losses (> 60%) were observed on chromosome arms 6q, 9p, and 18q 
      and the Y chromosome. Moderately frequent losses (40-60%) were observed on 
      chromosome arms 3p, 4q, 8p, and 21q. Interestingly, these samples showed 
      extremely high frequencies of increases in copy numbers of DNA sequences on the 
      long arm of chromosome 20 (15/18, 83%). We further analyzed five cell lines by 
      fluorescence in situ hybridization (FISH) with probes on chromosome 20 to define 
      the increase in copy number more accurately, and we found that 20q was increased 
      to between 5 and 8 copies per cell. These results suggest the existence of an 
      oncogene or oncogenes in 20q that play a role in the development and/or the 
      progression of pancreatic carcinogenesis.
FAU - Fukushige, S
AU  - Fukushige S
AD  - Department of Molecular Pathology, Tohoku University School of Medicine, Miyagi, 
      Japan.
FAU - Waldman, F M
AU  - Waldman FM
FAU - Kimura, M
AU  - Kimura M
FAU - Abe, T
AU  - Abe T
FAU - Furukawa, T
AU  - Furukawa T
FAU - Sunamura, M
AU  - Sunamura M
FAU - Kobari, M
AU  - Kobari M
FAU - Horii, A
AU  - Horii A
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - Genes Chromosomes Cancer
JT  - Genes, chromosomes & cancer
JID - 9007329
RN  - 0 (DNA, Neoplasm)
SB  - IM
MH  - Adenocarcinoma/*genetics/pathology
MH  - Adult
MH  - Aged
MH  - Chromosome Aberrations/*genetics
MH  - Chromosome Banding
MH  - Chromosome Mapping
MH  - Chromosomes, Human/genetics
MH  - Chromosomes, Human, Pair 20/*genetics
MH  - DNA, Neoplasm/analysis
MH  - Female
MH  - Humans
MH  - In Situ Hybridization, Fluorescence
MH  - Male
MH  - Metaphase
MH  - Middle Aged
MH  - Pancreatic Neoplasms/*genetics/pathology
MH  - Spectrometry, Fluorescence
MH  - Tumor Cells, Cultured
EDAT- 1997/07/01 00:00
MHDA- 2000/06/20 09:00
CRDT- 1997/07/01 00:00
PHST- 1997/07/01 00:00 [pubmed]
PHST- 2000/06/20 09:00 [medline]
PHST- 1997/07/01 00:00 [entrez]
AID - 10.1002/(SICI)1098-2264(199707)19:3<161::AID-GCC5>3.0.CO;2-W [pii]
AID - 10.1002/(sici)1098-2264(199707)19:3<161::aid-gcc5>3.0.co;2-w [doi]
PST - ppublish
SO  - Genes Chromosomes Cancer. 1997 Jul;19(3):161-9. doi: 
      10.1002/(sici)1098-2264(199707)19:3<161::aid-gcc5>3.0.co;2-w.