PMID- 9219168
OWN - NLM
STAT- MEDLINE
DCOM- 19970924
LR  - 20210518
IS  - 1046-6673 (Print)
IS  - 1046-6673 (Linking)
VI  - 8
IP  - 7
DP  - 1997 Jul
TI  - Suppression of anti-glomerular basement membrane nephritis by administration of 
      anti-monocyte chemoattractant protein-1 antibody in WKY rats.
PG  - 1174-8
AB  - Anti-glomerular basement membrane (GBM) nephritis in WKY rats is characterized by 
      an accumulation of CD8-positive lymphocytes (CD8+ lym) and monocytes/macrophages 
      (Mo/M psi) in the glomeruli and crescent formation. In the study presented here, 
      the involvement of a chemokine for Mo/M psi, monocyte chemoattractant protein-1 
      (MCP-1), was examined in this model. An intense induction of mRNA for MCP-1 in 
      the glomeruli and a suppressive effect of anti-MCP-1 antibody administration on 
      the glomerular Mo/M psi accumulation and proteinuria were found. MCP-1 mRNA was 
      expressed intensely in the glomeruli 4 d after the anti-GBM antibody injection. 
      When MCP-1 was neutralized with anti-MCP-1 antibody administration, the number of 
      Mo/M psi infiltrating in the glomeruli decreased by 34.7% (19.6 +/- 7.1 versus 
      30.0 +/- 6.0 per glomerular cross-section, P < 0.01) and proteinuria by 66.2% 
      (15.6 +/- 9.3 versus 46.1 +/- 15.1 mg/d, P < 0.01) at day 4. In contrast, the 
      number of CD8+ lym accumulating in the glomeruli was not affected significantly 
      (6.6 +/- 2.7 versus 6.0 +/- 3.0 per glomerular cross-section, P > 0.05). However, 
      the treatment with the anti-MCP-1 antibody did not reduce Mo/M psi infiltration, 
      urinary protein excretion, and crescent formation at day 8. These data suggest 
      that MCP-1 plays a role in the glomerular accumulation of Mo/M psi, and that the 
      infiltrating Mo/M psi cause glomerular injury and increased excretion of protein 
      in the urine.
FAU - Fujinaka, H
AU  - Fujinaka H
AD  - Department of Pathology, Niigata University School of Medicine, Japan.
FAU - Yamamoto, T
AU  - Yamamoto T
FAU - Takeya, M
AU  - Takeya M
FAU - Feng, L
AU  - Feng L
FAU - Kawasaki, K
AU  - Kawasaki K
FAU - Yaoita, E
AU  - Yaoita E
FAU - Kondo, D
AU  - Kondo D
FAU - Wilson, C B
AU  - Wilson CB
FAU - Uchiyama, M
AU  - Uchiyama M
FAU - Kihara, I
AU  - Kihara I
LA  - eng
GR  - DK 20043/DK/NIDDK NIH HHS/United States
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - United States
TA  - J Am Soc Nephrol
JT  - Journal of the American Society of Nephrology : JASN
JID - 9013836
RN  - 0 (Antibodies, Monoclonal)
RN  - 0 (Chemokine CCL2)
RN  - 0 (RNA, Messenger)
SB  - IM
MH  - Animals
MH  - Antibodies, Monoclonal
MH  - Basement Membrane/immunology
MH  - Chemokine CCL2/*antagonists & inhibitors/genetics/immunology
MH  - Gene Expression
MH  - Glomerulonephritis/etiology/immunology/*prevention & control
MH  - Kidney Glomerulus/immunology/pathology
MH  - Macrophages/immunology/pathology
MH  - Male
MH  - Monocytes/immunology/pathology
MH  - Neutralization Tests
MH  - Proteinuria/etiology/prevention & control
MH  - RNA, Messenger/genetics/metabolism
MH  - Rats
MH  - Rats, Inbred WKY
EDAT- 1997/07/01 00:00
MHDA- 1997/07/01 00:01
CRDT- 1997/07/01 00:00
PHST- 1997/07/01 00:00 [pubmed]
PHST- 1997/07/01 00:01 [medline]
PHST- 1997/07/01 00:00 [entrez]
AID - 10.1681/ASN.V871174 [doi]
PST - ppublish
SO  - J Am Soc Nephrol. 1997 Jul;8(7):1174-8. doi: 10.1681/ASN.V871174.