PMID- 9219566
OWN - NLM
STAT- MEDLINE
DCOM- 19970805
LR  - 20190702
IS  - 0027-5107 (Print)
IS  - 0027-5107 (Linking)
VI  - 386
IP  - 3
DP  - 1997 Jun
TI  - Disruption of microtubule assembly and spindle formation as a mechanism for the 
      induction of aneuploid cells by sodium arsenite and vanadium pentoxide.
PG  - 291-8
AB  - Arsenic and vanadium are important environmental and industrial pollutants. Due 
      to their widespread occurrence and potential genotoxicity, we studied the 
      aneuploidy-inducing effects of these elements in cultured human lymphocytes using 
      a variety of techniques including fluorescence in situ hybridization (FISH) with 
      DNA probes for chromosomes 1 and 7, immunostaining of the lymphocyte spindle 
      apparatus, and an in vitro assay measuring the polymerization and 
      depolymerization of tubulin. Dose-related increases in hyperdiploidy were seen in 
      lymphocyte cultures treated with sodium arsenite (NaAsO2) or vanadium pentoxide 
      (V2O5) over concentrations ranging from 0.001 to 0.1 microM. NaAsO2-treated cells 
      from different donors exhibited similar hyperdiploid frequencies, whereas 
      substantial inter-individual variability was seen in the V2O5-treated cells. 
      Examination of the spindle apparatus using an anti-beta-tubulin antibody 
      indicated that these compounds might disrupt spindle formation by interacting 
      with microtubules. Additional in vitro assays using purified tubulin indicated 
      that both compounds inhibited microtubule assembly and induced tubulin 
      depolymerization. These results indicate that in vitro exposure to both NaAsO2 
      and V2O5 can induce aneuploidy in human lymphocytes, and that this effect may 
      occur through a disruption of microtubule function.
FAU - Ramirez, P
AU  - Ramirez P
AD  - Department of Genetics and Environmental Toxicology, Instituto de Investigaciones 
      Biomedicas, U.N.A.M., Mexico, DF, Mexico.
FAU - Eastmond, D A
AU  - Eastmond DA
FAU - Laclette, J P
AU  - Laclette JP
FAU - Ostrosky-Wegman, P
AU  - Ostrosky-Wegman P
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - Netherlands
TA  - Mutat Res
JT  - Mutation research
JID - 0400763
RN  - 0 (Arsenites)
RN  - 0 (Sodium Compounds)
RN  - 0 (Vanadium Compounds)
RN  - 48OVY2OC72 (sodium arsenite)
RN  - BVG363OH7A (vanadium pentoxide)
SB  - IM
MH  - *Aneuploidy
MH  - Arsenites/*toxicity
MH  - Cells, Cultured
MH  - Dimerization
MH  - Female
MH  - Humans
MH  - Lymphocytes/*drug effects/pathology/ultrastructure
MH  - Male
MH  - Microtubules/*drug effects/ultrastructure
MH  - Sodium Compounds/*toxicity
MH  - Vanadium Compounds/*toxicity
EDAT- 1997/06/01 00:00
MHDA- 1997/06/01 00:01
CRDT- 1997/06/01 00:00
PHST- 1997/06/01 00:00 [pubmed]
PHST- 1997/06/01 00:01 [medline]
PHST- 1997/06/01 00:00 [entrez]
AID - S1383-5742(97)00018-5 [pii]
AID - 10.1016/s1383-5742(97)00018-5 [doi]
PST - ppublish
SO  - Mutat Res. 1997 Jun;386(3):291-8. doi: 10.1016/s1383-5742(97)00018-5.