PMID- 9219582
OWN - NLM
STAT- MEDLINE
DCOM- 19970805
LR  - 20190702
IS  - 0027-5107 (Print)
IS  - 0027-5107 (Linking)
VI  - 377
IP  - 1
DP  - 1997 Jun 9
TI  - Induction of micronuclei, hyperdiploidy and chromosomal breakage affecting the 
      centric/pericentric regions of chromosomes 1 and 9 in human amniotic fluid cells 
      after treatment with asbestos and ceramic fibers.
PG  - 77-87
AB  - This article describes the induction of micronuclei, hyperdiploidy and chromosome 
      breakage in human amniotic cells in vitro by amosite, chrysotile and crocidolite 
      asbestos, and ceramic fibers. The response of human (amniotic fluid cells) and 
      rodent (Syrian hamster embryo fibroblasts, SHE) cells to fiber treatment was 
      compared using the micronucleus assay. The data of the rodent studies were taken 
      from a previous investigation (Dopp, E. et al. (1995) Environ. Health Perspect., 
      103, 268-271). All types of mineral fibers caused a significant increase of 
      micronucleated cells. The kinetochore analysis revealed that all three types of 
      asbestos and ceramic fibers yielded similar effects. Approximately 50% of the 
      induced micronuclei were kinetochore-negative indicating formation through 
      clastogenic events. Human amniotic cells were much less susceptible than SHE 
      cells to the induction of micronuclei by mineral fibers. This again demonstrates 
      that SHE cells are more susceptible to chromosomal changes than human amniotic 
      fluid cells. The application of fluorescence in situ hybridization (FISH) with 
      tandem DNA probes yielded more detailed information about specific structural 
      chromosome aberrations in the 1 (cen-q12) and 9 (cen-q12) regions and about 
      abnormal numbers of chromosomes in interphase human amniotic fluid cells. Using 
      this FISH approach we found a statistically significant increase of chromosomal 
      breakage in the pericentric heterochromatin regions of chromosomes 1 and 9 in 
      interphase human amniotic cells after exposure to asbestos and ceramic fibers 
      compared to control cells. The number of hyperdiploid cells was also 
      significantly increased. Our results show that asbestos fibers as well as ceramic 
      fibers are inducers of structural and numerical chromosomal aberrations in human 
      amniotic fluid cells.
FAU - Dopp, E
AU  - Dopp E
AD  - Department of Biology, University of Rostock, Germany.
FAU - Schuler, M
AU  - Schuler M
FAU - Schiffmann, D
AU  - Schiffmann D
FAU - Eastmond, D A
AU  - Eastmond DA
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - Netherlands
TA  - Mutat Res
JT  - Mutation research
JID - 0400763
RN  - 0 (DNA Probes)
RN  - 0 (Mineral Fibers)
RN  - 1332-21-4 (Asbestos)
SB  - IM
MH  - Amniotic Fluid/cytology
MH  - Animals
MH  - Asbestos/*toxicity
MH  - Cell Nucleus/drug effects
MH  - Cells, Cultured
MH  - Ceramics/*toxicity
MH  - *Chromosome Aberrations
MH  - Chromosome Breakage
MH  - *Chromosomes, Human, Pair 1
MH  - *Chromosomes, Human, Pair 9
MH  - Cricetinae
MH  - DNA Probes
MH  - Diploidy
MH  - Humans
MH  - In Situ Hybridization, Fluorescence
MH  - Kinetochores/drug effects
MH  - Mesocricetus
MH  - Micronuclei, Chromosome-Defective
MH  - Microscopy, Fluorescence
MH  - Mineral Fibers/toxicity
EDAT- 1997/06/09 00:00
MHDA- 1997/06/09 00:01
CRDT- 1997/06/09 00:00
PHST- 1997/06/09 00:00 [pubmed]
PHST- 1997/06/09 00:01 [medline]
PHST- 1997/06/09 00:00 [entrez]
AID - S0027-5107(97)00062-6 [pii]
AID - 10.1016/s0027-5107(97)00062-6 [doi]
PST - ppublish
SO  - Mutat Res. 1997 Jun 9;377(1):77-87. doi: 10.1016/s0027-5107(97)00062-6.