PMID- 9220600
OWN - NLM
STAT- MEDLINE
DCOM- 19970912
LR  - 20190831
IS  - 0378-1135 (Print)
IS  - 0378-1135 (Linking)
VI  - 55
IP  - 1-4
DP  - 1997 Apr
TI  - Effect of maternally acquired Aujeszky's disease (pseudorabies) virus-specific 
      antibody in pigs on establishment of latency and seroconversion to differential 
      glycoproteins after low dose challenge.
PG  - 91-8
AB  - This study investigated whether (1) passively immune pigs could become latently 
      infected after challenge with low doses of wild type pseudorabies virus (PRV) and 
      (2) if seroconversion to PRV could be consistently detected using two 
      commercially available differential diagnostic ELISAs. Three litters of piglets 
      with passively acquired PRV serum neutralizing (SN) antibody (geometric mean 
      titers 47.03 to 95.10) were challenged at 6 to 12 days of age with 236 to 500 
      TCID50 of Shope strain virus; pigs were vaccinated at 11 weeks of age with a 
      commercially available genetically engineered vaccine (TK- gE- gG- Iowa S62 
      strain PRV). Vaccination was intended to reduce the risk of reactivation of 
      latent infection resulting in spread of virulent PRV infection to previously 
      uninfected pigs during the experiment. Vaccination at this age also approximated 
      common field practices in infected herds. After 15 weeks, all challenged pigs 
      were seropositive on the PRV glycoprotein (g or gp) E differential ELISA but were 
      seronegative on the gG differential ELISA. All three challenge groups had pigs 
      that were latently infected as evidenced by the detection of PRV DNA by 
      polymerase chain reaction (PCR) assay of their trigeminal ganglia (TG). There was 
      a significant inverse relationship observed for age at challenge and the 
      proportion of PCR positive pigs in the group 15 weeks postchallenge (p = 0.0004). 
      This trend was independent of the passively acquired PRV SN antibody titers at 
      challenge. In this study, passively acquired antibody did not provide protection 
      against establishment of latent infection in piglets after exposure to low doses 
      of virulent PRV. These latent infections were detected serologically by only one 
      of two available differential diagnostic ELISA.
FAU - McCaw, M B
AU  - McCaw MB
AD  - Food Animal and Equine Medicine Dept., College of Veterinary Medicine, North 
      Carolina State University, Raleigh 27606, USA. mmccaw@sn1.cvm.ncsu.edu
FAU - Osorio, F A
AU  - Osorio FA
FAU - Wheeler, J
AU  - Wheeler J
FAU - Xu, J
AU  - Xu J
FAU - Erickson, G A
AU  - Erickson GA
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - Netherlands
TA  - Vet Microbiol
JT  - Veterinary microbiology
JID - 7705469
RN  - 0 (Antibodies, Viral)
RN  - 0 (DNA Primers)
SB  - IM
MH  - Analysis of Variance
MH  - Animals
MH  - Antibodies, Viral/*blood
MH  - DNA Primers
MH  - Diagnosis, Differential
MH  - Enzyme-Linked Immunosorbent Assay/methods
MH  - Female
MH  - Herpesvirus 1, Suid/isolation & purification/*physiology
MH  - *Immunity, Maternally-Acquired
MH  - Polymerase Chain Reaction/methods
MH  - Pseudorabies/blood/diagnosis/*immunology
MH  - Swine
MH  - Virus Latency
EDAT- 1997/04/01 00:00
MHDA- 1997/04/01 00:01
CRDT- 1997/04/01 00:00
PHST- 1997/04/01 00:00 [pubmed]
PHST- 1997/04/01 00:01 [medline]
PHST- 1997/04/01 00:00 [entrez]
AID - S0378-1135(96)01315-6 [pii]
AID - 10.1016/s0378-1135(96)01315-6 [doi]
PST - ppublish
SO  - Vet Microbiol. 1997 Apr;55(1-4):91-8. doi: 10.1016/s0378-1135(96)01315-6.