PMID- 9220618 OWN - NLM STAT- MEDLINE DCOM- 19970912 LR - 20220321 IS - 0378-1135 (Print) IS - 0378-1135 (Linking) VI - 55 IP - 1-4 DP - 1997 Apr TI - Porcine reproductive and respiratory syndrome virus: a persistent infection. PG - 231-40 AB - Persistent infection with porcine reproductive and respiratory syndrome virus (PRRSV) was shown in experimentally infected pigs by isolation of virus from oropharyngeal samples for up to 157 days after challenge. Four 4 week old, conventional, PRRSV antibody-negative pigs were intranasally inoculated with PRRSV (ATCC VR-2402). Serum samples were collected every 2 to 3 days until day 42 post inoculation (PI), then approximately every 14 days until day 213 PI. Fecal samples were collected at the time of serum collection through day 35 PI. Oropharyngeal samples were collected at the time of serum collection from 56 to 213 days PI by scraping the oropharyngeal area with a sterile spoon, especially targeting the palatine tonsil. Turbinate, tonsil, lung, parotid salivary gland, spleen, lymph nodes and serum were collected postmortem on day 220 PI. Virus isolation (VI) on porcine alveolar macrophage cultures was attempted on all serum, fecal and oropharyngeal samples, as well as tissues collected postmortem. Postmortem tonsil tissues and selected fecal samples were also assayed for the presence of PRRSV RNA by the polymerase chain reaction (PCR). Serum antibody titers were determined by IFA, ELISA and SVN. Virus was isolated from all serum samples collected on days 2 to 11 PI and intermittently for up to 23 days in two pigs. No PRRSV was isolated from fecal samples, but 3 of 24 samples were PCR positive, suggesting the presence of inactivated virus. Oropharyngeal samples from each pig were VI positive 1 or more times between 56 and 157 days PI. Oropharyngeal samples from 3 of 4 pigs were VI positive on days 56, 70 and 84 PI. Virus was isolated from one pig on day 157 PI, 134 days after the last isolation of virus from serum from this animal. Virus was isolated from oropharyngeal samples for several weeks after the maximum serum antibody response, as measured by IFA, ELISA and SVN tests. All tissues collected postmortem were VI negative and postmortem tonsil samples were also negative by PCR. An important element in the transmission of PRRSV is the duration of virus shedding. The results of this study provided direct evidence of persistent PRRSV infection and explain field observations of long-term herd infection and transmission via purchase of clinically normal, but PRRSV infected, animals. Effective prevention and control strategies will need to be developed in the context of these results. FAU - Wills, R W AU - Wills RW AD - Veterinary Diagnostic Laboratory, College of Veterinary Medicine, Iowa State University, Ames 50011-1250, USA. rwwills@iastate.edu FAU - Zimmerman, J J AU - Zimmerman JJ FAU - Yoon, K J AU - Yoon KJ FAU - Swenson, S L AU - Swenson SL FAU - McGinley, M J AU - McGinley MJ FAU - Hill, H T AU - Hill HT FAU - Platt, K B AU - Platt KB FAU - Christopher-Hennings, J AU - Christopher-Hennings J FAU - Nelson, E A AU - Nelson EA LA - eng PT - Journal Article PT - Research Support, Non-U.S. Gov't PL - Netherlands TA - Vet Microbiol JT - Veterinary microbiology JID - 7705469 RN - 0 (Antibodies, Viral) SB - IM MH - Animals MH - Antibodies, Viral/blood MH - Enzyme-Linked Immunosorbent Assay MH - Feces/virology MH - Macrophages, Alveolar/*virology MH - Oropharynx/*virology MH - Porcine Reproductive and Respiratory Syndrome/immunology/*physiopathology MH - Porcine respiratory and reproductive syndrome virus/*isolation & purification MH - Swine MH - Time Factors MH - Virus Shedding EDAT- 1997/04/01 00:00 MHDA- 1997/04/01 00:01 CRDT- 1997/04/01 00:00 PHST- 1997/04/01 00:00 [pubmed] PHST- 1997/04/01 00:01 [medline] PHST- 1997/04/01 00:00 [entrez] AID - S0378-1135(96)01337-5 [pii] AID - 10.1016/s0378-1135(96)01337-5 [doi] PST - ppublish SO - Vet Microbiol. 1997 Apr;55(1-4):231-40. doi: 10.1016/s0378-1135(96)01337-5.