PMID- 9220623
OWN - NLM
STAT- MEDLINE
DCOM- 19970912
LR  - 20190831
IS  - 0378-1135 (Print)
IS  - 0378-1135 (Linking)
VI  - 55
IP  - 1-4
DP  - 1997 Apr
TI  - Field isolates of porcine reproductive and respiratory syndrome virus (PRRSV) 
      vary in their susceptibility to antibody dependent enhancement (ADE) of 
      infection.
PG  - 277-87
AB  - Seventeen porcine reproductive and respiratory syndrome virus (PRRSV) field 
      isolates, including isolate ISU-P, were evaluated for their susceptibility to 
      antibody dependent enhancement (ADE) of infection mediated by antibodies raised 
      against PRRSV isolate ISU-P. Progeny virus yields of ISU-P and 4 of 16 field 
      isolates in porcine alveolar macrophages (PAM) were reduced following treatment 
      with a concentration of antibody that neutralized ISU-P (p < 0.01). In contrast, 
      the yields of 12 of 17 field isolates were enhanced (p < 0.01). Treatment of all 
      isolates with a 10-fold lower concentration of this antibody significantly (p < 
      0.01) increased virus yields of all isolates in PAM. However, the degree of 
      enhancement varied among the isolates when compared to the enhancement of the 
      yield of ISU-P. While no differences in enhancement were observed among ISU-P and 
      9 field isolates, yield enhancement of 6 and 1 isolates were less than and more 
      than the yield enhancement of ISU-P, respectively (p < 0.05). The degree of 
      enhancement mediated by a high concentration of antibody raised against ISU-P was 
      inversely proportional to the ability of the antibody to neutralize the isolates 
      (r = 0.92). In contrast, no direct correlation (r = 0.32) was observed between 
      the degree of enhancement mediated by a low concentration of antibody and the 
      ability of the antibody to neutralize the isolates. These data suggest that the 
      variability in the susceptibility of PRRSV isolates to ADE arise from 
      quantitative and/or qualitative differences in the antigenic determinants 
      associated with virus neutralization and/or ADE. The antigenic diversity and the 
      wide range in the susceptibility to ADE that exists among field isolates indicate 
      that ADE should be taken into consideration in the development of effective 
      immunization strategies for PRRS.
FAU - Yoon, K J
AU  - Yoon KJ
AD  - Veterinary Diagnostic Laboratory, College of Veterinary Medicine, Iowa State 
      University, Ames 50011, USA. kyoon@iastate.edu
FAU - Wu, L L
AU  - Wu LL
FAU - Zimmerman, J J
AU  - Zimmerman JJ
FAU - Platt, K B
AU  - Platt KB
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PT  - Research Support, U.S. Gov't, Non-P.H.S.
PL  - Netherlands
TA  - Vet Microbiol
JT  - Veterinary microbiology
JID - 7705469
RN  - 0 (Antibodies, Viral)
SB  - IM
MH  - Animals
MH  - Antibodies, Viral/*immunology
MH  - Antigenic Variation
MH  - Macrophages, Alveolar/*virology
MH  - Porcine Reproductive and Respiratory Syndrome/*immunology
MH  - Porcine respiratory and reproductive syndrome virus/*immunology/isolation & 
      purification
MH  - Regression Analysis
MH  - Swine
EDAT- 1997/04/01 00:00
MHDA- 1997/04/01 00:01
CRDT- 1997/04/01 00:00
PHST- 1997/04/01 00:00 [pubmed]
PHST- 1997/04/01 00:01 [medline]
PHST- 1997/04/01 00:00 [entrez]
AID - S0378-1135(96)01338-7 [pii]
AID - 10.1016/s0378-1135(96)01338-7 [doi]
PST - ppublish
SO  - Vet Microbiol. 1997 Apr;55(1-4):277-87. doi: 10.1016/s0378-1135(96)01338-7.