PMID- 9222108
OWN - NLM
STAT- MEDLINE
DCOM- 19970918
LR  - 20191102
IS  - 0196-4763 (Print)
IS  - 0196-4763 (Linking)
VI  - 28
IP  - 3
DP  - 1997 Jul 1
TI  - Flow cytometric analysis of micronucleus induction in rat bone marrow 
      polychromatic erythrocytes by 1,2;3,4-diepoxybutane, 3,4-epoxy-1-butene, and 
      1,2-epoxybutane-3,4-diol.
PG  - 228-35
AB  - Automation of the analysis of micronucleus induction with flow cytometry was 
      developed by using mouse bone marrow or peripheral blood. In the present study, 
      we report the use of flow cytometry for the identification and quantification of 
      micronuclei (MN) induced in rat bone marrow polychromatic erythrocytes. Three 
      metabolites of the industrial chemical 1,3-butadiene, namely 
      1,2;3,4-diepoxybutane (DEB), 3,4-epoxy-1-butene (EB) and 1,2-epoxybutane-3,4-diol 
      (diol-EB), were studied in addition to mitomycin C and cyclophosphamide, which 
      served as positive controls. DEB showed a dose-dependent increase in the 
      frequency of MN, whereas EB was completely negative and diol-EB only weakly 
      positive at one dose level. The effect of the positive control compounds was 
      observed 48 h after a single injection in a dose-dependent manner. Flow cytometry 
      was an effective method to quantitate bone marrow MN induction in the rat when 
      density gradient separation of polychromatic erythrocytes is used. The results 
      are compatible with the theory that oxidation of EB to the mutagenic metabolite 
      DEB occurs at a low rate in rat bone marrow and that EB is detoxified by epoxide 
      hydrolase and by conjugation with glutathione by glutathione transferase yielding 
      nonmutagenic metabolites. Thus, the reported lack of MN induction by 
      1,3-butadiene inhalation in rat bone marrow is explained.
FAU - Lahdetie, J
AU  - Lahdetie J
AD  - Department of Medical Genetics, University of Turku, Finland.
FAU - Grawe, J
AU  - Grawe J
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - Cytometry
JT  - Cytometry
JID - 8102328
RN  - 0 (Benzimidazoles)
RN  - 0 (Epoxy Compounds)
RN  - 0 (Fluorescent Dyes)
RN  - 0 (Glycols)
RN  - 0 (Mutagens)
RN  - 17177-50-3 (3,4-epoxybutane-1,2-diol)
RN  - 478ERR5NKR (3,4-epoxy-1-butene)
RN  - 60OB65YNAB (diepoxybutane)
RN  - P976261J69 (bisbenzimide ethoxide trihydrochloride)
SB  - IM
MH  - Animals
MH  - Benzimidazoles
MH  - *Bone Marrow Cells
MH  - Cell Separation
MH  - Epoxy Compounds/toxicity
MH  - Erythrocyte Aging
MH  - Erythrocytes/cytology/*drug effects
MH  - Flow Cytometry/*methods
MH  - Fluorescent Dyes
MH  - Glycols/toxicity
MH  - Male
MH  - Mice
MH  - *Micronucleus Tests
MH  - Mutagens/toxicity
MH  - Rats
MH  - Rats, Sprague-Dawley
EDAT- 1997/07/01 00:00
MHDA- 2000/06/20 09:00
CRDT- 1997/07/01 00:00
PHST- 1997/07/01 00:00 [pubmed]
PHST- 2000/06/20 09:00 [medline]
PHST- 1997/07/01 00:00 [entrez]
AID - 10.1002/(SICI)1097-0320(19970701)28:3<228::AID-CYTO7>3.0.CO;2-9 [pii]
AID - 10.1002/(sici)1097-0320(19970701)28:3<228::aid-cyto7>3.3.co;2-j [doi]
PST - ppublish
SO  - Cytometry. 1997 Jul 1;28(3):228-35. doi: 
      10.1002/(sici)1097-0320(19970701)28:3<228::aid-cyto7>3.3.co;2-j.