PMID- 9223332
OWN - NLM
STAT- MEDLINE
DCOM- 19970827
LR  - 20230331
IS  - 0027-8424 (Print)
IS  - 1091-6490 (Electronic)
IS  - 0027-8424 (Linking)
VI  - 94
IP  - 15
DP  - 1997 Jul 22
TI  - The hepatitis B virus X gene induces p53-mediated programmed cell death.
PG  - 8162-7
AB  - The human hepatitis B virus (HBV) protein pX is a multifunctional regulatory 
      protein that is known to affect both transcription and cell growth. Here we 
      describe induction of apoptosis in NIH 3T3 polyclonal cell lines upon stimulation 
      of pX expression from a dexamethasone inducible mouse mammary tumor virus 
      (MMTV)-X expression vector. The effect of long-term pX expression on the cell 
      survival of mouse fibroblasts was confirmed in colony generation assays. This 
      effect is not shared either by the other HBV products and it is c-myc mediated, 
      as shown by the use of a dominant negative deletion mutant of c-myc. pX also 
      sensitize cells to programmed cell death after exposure to DNA damaging agents. 
      Taking advantage of stable transfectants carrying the p53val135 
      temperature-sensitive allele, we directly demonstrate that induction of apoptosis 
      by pX requires p53. In p53 null mouse embryo fibroblasts pX activates 
      transcription and confers an evident growth advantage without loss of cell 
      viability. Although pX protein was not detectable in the experimental conditions 
      we used, our results indicate that its expression affects both cell growth and 
      cell death control.
FAU - Chirillo, P
AU  - Chirillo P
AD  - Fondazione Andrea Cesalpino, Policlinico Umberto I, Universita degli Studi di 
      Roma La Sapienza, 00161 Rome, Italy.
FAU - Pagano, S
AU  - Pagano S
FAU - Natoli, G
AU  - Natoli G
FAU - Puri, P L
AU  - Puri PL
FAU - Burgio, V L
AU  - Burgio VL
FAU - Balsano, C
AU  - Balsano C
FAU - Levrero, M
AU  - Levrero M
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - Proc Natl Acad Sci U S A
JT  - Proceedings of the National Academy of Sciences of the United States of America
JID - 7505876
RN  - 0 (Culture Media, Serum-Free)
RN  - 0 (Hepatitis B Antigens)
RN  - 0 (Trans-Activators)
RN  - 0 (Tumor Suppressor Protein p53)
RN  - 0 (Viral Regulatory and Accessory Proteins)
RN  - 0 (hepatitis B virus X protein)
SB  - IM
MH  - 3T3 Cells
MH  - Animals
MH  - Apoptosis/*genetics
MH  - Cell Survival/genetics
MH  - Culture Media, Serum-Free
MH  - DNA Replication/genetics
MH  - Gene Expression Regulation/*genetics
MH  - Hepatitis B Antigens/*genetics
MH  - Mice
MH  - Mice, Inbred BALB C
MH  - Trans-Activators/*genetics
MH  - Transcriptional Activation
MH  - Tumor Suppressor Protein p53/*genetics
MH  - Viral Regulatory and Accessory Proteins
PMC - PMC21574
EDAT- 1997/07/22 00:00
MHDA- 1997/07/22 00:01
PMCR- 1998/01/22
CRDT- 1997/07/22 00:00
PHST- 1997/07/22 00:00 [pubmed]
PHST- 1997/07/22 00:01 [medline]
PHST- 1997/07/22 00:00 [entrez]
PHST- 1998/01/22 00:00 [pmc-release]
AID - 1095 [pii]
AID - 10.1073/pnas.94.15.8162 [doi]
PST - ppublish
SO  - Proc Natl Acad Sci U S A. 1997 Jul 22;94(15):8162-7. doi: 
      10.1073/pnas.94.15.8162.