PMID- 9223345
OWN - NLM
STAT- MEDLINE
DCOM- 19970827
LR  - 20190501
IS  - 0027-8424 (Print)
IS  - 1091-6490 (Electronic)
IS  - 0027-8424 (Linking)
VI  - 94
IP  - 15
DP  - 1997 Jul 22
TI  - Specific requirement of putrescine for the mitogenic action of juvenile hormone 
      on adult insect neuroblasts.
PG  - 8238-42
AB  - Persistent neurogenesis in an adult insect brain was recently shown to be 
      stimulated by juvenile hormone (JH). This morphogenetic hormone was also shown to 
      act on polyamine biosynthesis. To analyze the possible involvement of polyamines 
      in the neurogenic action of JH, two series of experiments were carried out with 
      adult female crickets, Acheta domesticus: (i) inhibition of the first key enzyme 
      in polyamine biosynthesis, ornithine decarboxylase, with 
      alpha-difluoromethylornithine (alpha-DFMO), and examination of the effects of 
      this treatment on the neuroblast proliferation response to JH; and (ii) 
      examination of the effects of putrescine supplementation on the mitotic index of 
      JH-deprived and alpha-DFMO-treated females. In control females, alpha-DFMO 
      treatment, as well as JH deprivation, greatly reduced neuroblast proliferation. 
      Putrescine supplementation in alpha-DFMO-treated insects overcame the effects of 
      alpha-DFMO, and allowed for detection of putrescine in the neural tissue and 
      stimulation of brain neurogenesis. In JH-deprived females, alpha-DFMO treatment 
      completely prevented the stimulatory action of JH on neuroblast proliferation and 
      on brain putrescine levels. By contrast, putrescine feeding of JH-deprived 
      animals was able to mimic the stimulatory effect of JH: brain putrescine levels 
      increased and neuroblast proliferation was restored. To our knowledge, this 
      report demonstrates for the first time that in vivo administration of putrescine 
      can mimic the effects of a morphogenetic hormone on adult neuroblast 
      proliferation, and shows the importance of polyamines, especially putrescine, in 
      the transduction of JH message in neural tissue.
FAU - Cayre, M
AU  - Cayre M
AD  - Laboratoire de Neurobiologie, Centre National de la Recherche Scientifique, 31 
      chemin Joseph Aiguier, 13402 Marseille cedex 20, France. cayre@irlnb.cnrs-mrs.fr
FAU - Strambi, C
AU  - Strambi C
FAU - Charpin, P
AU  - Charpin P
FAU - Augier, R
AU  - Augier R
FAU - Strambi, A
AU  - Strambi A
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - Proc Natl Acad Sci U S A
JT  - Proceedings of the National Academy of Sciences of the United States of America
JID - 7505876
RN  - 0 (Enzyme Inhibitors)
RN  - 0 (Juvenile Hormones)
RN  - 0 (Mitogens)
RN  - 0 (Ornithine Decarboxylase Inhibitors)
RN  - V10TVZ52E4 (Putrescine)
RN  - ZQN1G5V6SR (Eflornithine)
SB  - IM
MH  - Animals
MH  - Brain/*drug effects/embryology
MH  - Eflornithine/pharmacology
MH  - Enzyme Inhibitors/pharmacology
MH  - Female
MH  - Gryllidae/*drug effects
MH  - Juvenile Hormones/antagonists & inhibitors/*pharmacology
MH  - Mitogens/antagonists & inhibitors/*pharmacology
MH  - Mitosis/drug effects
MH  - Neurons/cytology/drug effects
MH  - Ornithine Decarboxylase Inhibitors
MH  - Putrescine/*pharmacology
PMC - PMC21587
EDAT- 1997/07/22 00:00
MHDA- 1997/07/22 00:01
PMCR- 1998/01/22
CRDT- 1997/07/22 00:00
PHST- 1997/07/22 00:00 [pubmed]
PHST- 1997/07/22 00:01 [medline]
PHST- 1997/07/22 00:00 [entrez]
PHST- 1998/01/22 00:00 [pmc-release]
AID - 1488 [pii]
AID - 10.1073/pnas.94.15.8238 [doi]
PST - ppublish
SO  - Proc Natl Acad Sci U S A. 1997 Jul 22;94(15):8238-42. doi: 
      10.1073/pnas.94.15.8238.