PMID- 9224405
OWN - NLM
STAT- MEDLINE
DCOM- 19970806
LR  - 20071114
IS  - 0022-4804 (Print)
IS  - 0022-4804 (Linking)
VI  - 69
IP  - 2
DP  - 1997 May
TI  - The temporal expression of transforming growth factor-beta 1 in early 
      aortocoronary vein grafts.
PG  - 349-53
AB  - The success of coronary reconstructive procedures is limited by the high 
      incidence of restenosis secondary to intimal hyperplasia (IH). Transforming 
      growth factor-beta 1 (TGF-beta 1) is a growth factor which has been shown to be 
      important in the early development of IH in arteries and peripheral vein grafts. 
      To date, there is little information concerning the early remodeling in 
      aortocoronary vein grafts (ACVG). The purpose of this study was to characterize 
      the expression of TGF-beta 1 expression in early aortocoronary vein grafts. 
      Eighteen mongrel dogs underwent aortocoronary vein bypass grafting. Vein grafts 
      were excised at 2 hr, 4 hr, and 7 days after implantation, snap frozen, and 
      processed for ribonuclease protection assays (RPA) using 32P-labeled riboprobes 
      for TGF-beta 1 and 18 S rRNA. TGF-beta 1 expression was quantified by 
      densitometric analysis of autoradiographs which were expressed as a ratio 
      TGF-beta 1/rRNA. Representative vessel rings were also collected for histology. 
      There was a significant rise in TGF-beta 1 expression in the 2-hr vein grafts 
      (0.42 +/- 0.04 compared to control saphenous vein (0.21 +/- 0.05, P < 0.02). In 
      addition, there was significant downregulation of TGF-beta 1 at 4 hr (0.28 +/- 
      0.05) and at 7 days (0.18 +/- 0.01) when compared to 2 hr (P < 0.05). 
      Histological specimens showed minimal intimal hyperplasia at 7 days. These 
      results show for the first time an acute rise in TGF-beta 1 expression in ACVG. 
      This upregulation quickly subsides by 4 hr and gene expression approaches control 
      values by 7 days. By understanding this temporal relationship of expression one 
      could better target potential therapeutic modalities to attenuate IH.
FAU - Dattilo, J B
AU  - Dattilo JB
AD  - Department of Surgery, Medical College of Virginia/Virginia Commonwealth 
      University, Richmond 27858, USA.
FAU - Lust, R M
AU  - Lust RM
FAU - Dattilo, M P
AU  - Dattilo MP
FAU - Parker, F M
AU  - Parker FM
FAU - Meadows, W M Jr
AU  - Meadows WM Jr
FAU - Sun, Y S
AU  - Sun YS
FAU - Chitwood, W R Jr
AU  - Chitwood WR Jr
FAU - Makhoul, R G
AU  - Makhoul RG
LA  - eng
GR  - HL 09301-01A1/HL/NHLBI NIH HHS/United States
PT  - Journal Article
PT  - Research Support, U.S. Gov't, Non-P.H.S.
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - United States
TA  - J Surg Res
JT  - The Journal of surgical research
JID - 0376340
RN  - 0 (Transforming Growth Factor beta)
SB  - IM
MH  - Animals
MH  - *Coronary Artery Bypass
MH  - Coronary Circulation
MH  - Dogs
MH  - Hyperplasia
MH  - Saphenous Vein
MH  - Time Factors
MH  - Transforming Growth Factor beta/*metabolism
MH  - Tunica Intima/pathology
MH  - Veins/*metabolism
EDAT- 1997/05/01 00:00
MHDA- 2001/03/28 10:01
CRDT- 1997/05/01 00:00
PHST- 1997/05/01 00:00 [pubmed]
PHST- 2001/03/28 10:01 [medline]
PHST- 1997/05/01 00:00 [entrez]
AID - S0022-4804(97)95076-9 [pii]
AID - 10.1006/jsre.1997.5076 [doi]
PST - ppublish
SO  - J Surg Res. 1997 May;69(2):349-53. doi: 10.1006/jsre.1997.5076.