PMID- 9225629
OWN - NLM
STAT- MEDLINE
DCOM- 19970806
LR  - 20101118
IS  - 0300-8835 (Print)
IS  - 0300-8835 (Linking)
VI  - 164
DP  - 1997
TI  - Clinical aspects on the use of preimplantation genetic diagnosis in couples at 
      risk.
PG  - 19-21
AB  - BACKGROUND: Preimplantation genetic diagnosis (PGD) is a new technique which may 
      become an attractive alternative to traditional prenatal diagnosis for couples at 
      risk of getting children with severe genetic diseases. We here report our 
      experience after the first trials in Sweden. METHODS: On day three after 
      fertilization by intracytoplasmic sperm injection (ICSI) one or two blastomeres 
      were biopsied and diagnosed by fluorescence in situ hybridization (FISH). Gender 
      determination was done in two cases where the female in the couples were carrier 
      of a severe X-chromosome bound disease (Wiskott-Aldrich disease or ornithine 
      transcarbamylase deficiency). RESULTS: The first couple got an embryo transfer in 
      each of their two treatment cycles. Two female embryos were transferred in the 
      first cycle and one in the second cycle. The second couple did not get any embryo 
      transfer in their two cycles. No clinical pregnancy occurred. CONCLUSION: With 
      the rapidly improving knowledge about inherited disease and refinement of the IVF 
      techniques, PGD will play an important clinical role in high risk groups within a 
      decade for both mono- and polygenic disorders. Both technical as well as legal 
      and ethical problems have however to be solved before these new techniques can be 
      applied on large scale.
FAU - Hanson, C
AU  - Hanson C
AD  - Department of Obstetrics and Gynecology, Goteborg University, Sweden.
FAU - Hamberger, L
AU  - Hamberger L
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - Denmark
TA  - Acta Obstet Gynecol Scand Suppl
JT  - Acta obstetricia et gynecologica Scandinavica. Supplement
JID - 0337655
SB  - IM
MH  - *Embryonic Development
MH  - Female
MH  - *Fertilization in Vitro/methods
MH  - Genetic Diseases, Inborn/*diagnosis
MH  - Genetic Linkage
MH  - Humans
MH  - In Situ Hybridization, Fluorescence
MH  - Male
MH  - Microinjections
MH  - Pregnancy
MH  - *Prenatal Diagnosis
MH  - Risk Factors
MH  - Spermatozoa
MH  - *X Chromosome
EDAT- 1997/01/01 00:00
MHDA- 1997/01/01 00:01
CRDT- 1997/01/01 00:00
PHST- 1997/01/01 00:00 [pubmed]
PHST- 1997/01/01 00:01 [medline]
PHST- 1997/01/01 00:00 [entrez]
PST - ppublish
SO  - Acta Obstet Gynecol Scand Suppl. 1997;164:19-21.