PMID- 9225681
OWN - NLM
STAT- MEDLINE
DCOM- 19970812
LR  - 20121115
IS  - 0364-5134 (Print)
IS  - 0364-5134 (Linking)
VI  - 42
IP  - 1
DP  - 1997 Jul
TI  - Cytokine profile of myelin basic protein-reactive T cells in multiple sclerosis 
      and healthy individuals.
PG  - 18-27
AB  - Myelin basic protein (MBP)-reactive T cells have been implicated in the 
      autoimmune pathogenesis of multiple sclerosis (MS). In this study, we examined 
      the cytokine profile of 531 primary MBP-reactive T-cell lines and 72 
      independently established clones from 32 patients with MS and 18 healthy controls 
      (NS) by using highly sensitive enzyme-linked immunosorbent assays. An increased 
      number of primary T-cell lines producing interferon-gamma (IFN gamma) and/or 
      interleukin-4 (IL-4) in response to MBP were found in patients with MS compared 
      with controls. No distinct Th1 or Th2 subtypes could be demonstrated among the 
      MBP-reactive clones. IL-4 was more frequently observed among MS-derived clones. 
      Clones derived from MS patients produced increased levels of IL-2, IL-4, tumor 
      necrosis factor-alpha (TNF alpha), IFN gamma, and IL-10, but not IL-6. It is 
      interesting that MBP-reactive T cells from MS patients expressing the 
      disease-associated HLA-DRB1*15 allele produced increased quantities of TNF alpha, 
      a cytokine suggested to play an important role in inflammation and demyelination. 
      When challenged with either MBP or a bacterial superantigen, the clones expressed 
      similar levels of the proinflammatory cytokine IFN gamma. Our study suggests a 
      functional difference in T-cell responses to MBP in patients with MS compared 
      with healthy individuals, and provides further insights into the role of 
      MBP-reactive T cells and their cytokine profile in the inflammatory processes of 
      MS.
FAU - Hermans, G
AU  - Hermans G
AD  - Multiple Sclerosis Research Unit, Dr L. Willems-Instituut and Limburgs 
      Universitair Centrum, Diepenbeek, Belgium.
FAU - Stinissen, P
AU  - Stinissen P
FAU - Hauben, L
AU  - Hauben L
FAU - Van den Berg-Loonen, E
AU  - Van den Berg-Loonen E
FAU - Raus, J
AU  - Raus J
FAU - Zhang, J
AU  - Zhang J
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - Ann Neurol
JT  - Annals of neurology
JID - 7707449
RN  - 0 (Cytokines)
RN  - 0 (Enterotoxins)
RN  - 0 (HLA Antigens)
RN  - 0 (Myelin Basic Protein)
RN  - 39424-53-8 (enterotoxin B, staphylococcal)
SB  - IM
MH  - Adult
MH  - Cell Line
MH  - Clone Cells
MH  - Cytokines/*metabolism
MH  - Enterotoxins/pharmacology
MH  - Female
MH  - HLA Antigens/genetics
MH  - Haplotypes
MH  - Humans
MH  - Kinetics
MH  - Male
MH  - Middle Aged
MH  - Multiple Sclerosis/*metabolism
MH  - Myelin Basic Protein/*pharmacology
MH  - Reference Values
MH  - T-Lymphocytes/*drug effects/*metabolism
EDAT- 1997/07/01 00:00
MHDA- 1997/07/01 00:01
CRDT- 1997/07/01 00:00
PHST- 1997/07/01 00:00 [pubmed]
PHST- 1997/07/01 00:01 [medline]
PHST- 1997/07/01 00:00 [entrez]
AID - 10.1002/ana.410420106 [doi]
PST - ppublish
SO  - Ann Neurol. 1997 Jul;42(1):18-27. doi: 10.1002/ana.410420106.