PMID- 9225857
OWN - NLM
STAT- MEDLINE
DCOM- 19970808
LR  - 20231213
IS  - 0167-4412 (Print)
IS  - 0167-4412 (Linking)
VI  - 34
IP  - 3
DP  - 1997 Jun
TI  - Footprinting of the spinach nitrite reductase gene promoter reveals the 
      preservation of nitrate regulatory elements between fungi and higher plants.
PG  - 465-76
AB  - Nitrite reductase (NiR) is the second enzyme in the nitrate assimilatory pathway 
      reducing nitrite to ammonium. The expression of the NiR gene is induced upon the 
      addition of nitrate. In an earlier study, a 130 bp upstream region of the spinach 
      NiR gene promoter, located between -330 to - 200, was shown to be necessary for 
      nitrate induction of beta-glucuronidase (GUS) expression in tissue-specific 
      manner in transgenic tobacco plant [28]. To further delineate the cis-acting 
      elements involved in nitrate regulation of NiR gene expression, transgenic 
      tobacco plants were generated with 5' deletions in the -330 to -200 region of the 
      spinach NiR gene promoter fused to the GUS gene. Plants with the NiR promoter 
      deleted to -230 showed a considerable increase in GUS activity in the presence of 
      nitrate, indicating that the 30 bp region between -230 to -200 is crucial for 
      nitrate-regulated expression of NiR. In vivo DMS footprinting of the -300 to -130 
      region of the NiR promoter in leaf tissues from two independent transgenic lines 
      revealed several nitrate-inducible footprints. Footprinting within the -230 to 
      -181 region revealed factor binding to two adjacent GATA elements separated by 24 
      bp. This arrangement of GATA elements is analogous to cis-regulatory sequences 
      found in the promoters of nitrate-inducible genes of Neurospora crassa, regulated 
      by the NIT2 Zn-finger protein. The -240 to -110 fragment of the NiR promoter, 
      which contains two NIT2 consensus core elements, bound in vitro to a fusion 
      protein comprising the zinc finger domain of the N. crassa NIT2 protein. The data 
      presented here show that nitrate-inducible expression of the NiR gene is mediated 
      by nitrate-specific binding of trans-acting factors to sequences preserved 
      between fungi and higher plants.
FAU - Rastogi, R
AU  - Rastogi R
AD  - Department of Molecular Biology & Genetics, University of Guelph, Ontario, 
      Canada.
FAU - Bate, N J
AU  - Bate NJ
FAU - Sivasankar, S
AU  - Sivasankar S
FAU - Rothstein, S J
AU  - Rothstein SJ
LA  - eng
PT  - Comparative Study
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - Netherlands
TA  - Plant Mol Biol
JT  - Plant molecular biology
JID - 9106343
RN  - 0 (DNA Primers)
RN  - 0 (Recombinant Fusion Proteins)
RN  - EC 1.7.- (Nitrite Reductases)
RN  - EC 2.5.1.18 (Glutathione Transferase)
RN  - EC 3.2.1.31 (Glucuronidase)
SB  - IM
MH  - Base Sequence
MH  - Conserved Sequence
MH  - DNA Footprinting
MH  - DNA Primers
MH  - Glucuronidase/biosynthesis
MH  - Glutathione Transferase/biosynthesis
MH  - Molecular Sequence Data
MH  - Neurospora crassa/genetics
MH  - Nitrite Reductases/biosynthesis/*genetics
MH  - Plants/genetics
MH  - Plants, Genetically Modified
MH  - Plants, Toxic
MH  - Polymerase Chain Reaction
MH  - *Promoter Regions, Genetic
MH  - Recombinant Fusion Proteins/biosynthesis
MH  - *Regulatory Sequences, Nucleic Acid
MH  - Sequence Deletion
MH  - Spinacia oleracea/*enzymology/*genetics
MH  - Nicotiana
EDAT- 1997/06/01 00:00
MHDA- 1997/06/01 00:01
CRDT- 1997/06/01 00:00
PHST- 1997/06/01 00:00 [pubmed]
PHST- 1997/06/01 00:01 [medline]
PHST- 1997/06/01 00:00 [entrez]
AID - 10.1023/a:1005842812321 [doi]
PST - ppublish
SO  - Plant Mol Biol. 1997 Jun;34(3):465-76. doi: 10.1023/a:1005842812321.