PMID- 9225958
OWN - NLM
STAT- MEDLINE
DCOM- 19970807
LR  - 20171116
IS  - 0344-5704 (Print)
IS  - 0344-5704 (Linking)
VI  - 40
IP  - 4
DP  - 1997
TI  - Methotrexate increases red blood cell concentrations of 6-methylmercaptopurine 
      ribonucleotide in rats in vivo.
PG  - 367-70
AB  - PURPOSE: To elucidate the effect of methotrexate (MTX) on 6-mercaptopurine (6-MP) 
      metabolism in rats. METHODS: Fourteen rats were given 6-MP 20 mg/kg daily for 7 
      days. Seven of the rats were also given MTX 20 mg/kg on days 5 and 7. Blood 
      samples were obtained from all rats on days 0.5 and 8, and red blood cell (RBC) 
      lysates were analysed for thiopurine methyltransferase (TPMT) activity and the 
      concentration of methylated 6-MP metabolites [methyl mercaptopurine 
      ribonucleotides (MMPRP)] and 6-thioguanine nucleotides (6-TGN). RESULTS: The 
      concentration of MMPRP increased 2.4 times from day 5 to day 8 in RBCs from rats 
      given MTX in addition to 6-MP, as against 1.2 times in rats given 6-MP alone (P = 
      0.003). 6-TGN levels increased and TPMT activity decreased from day 5 to day 8, 
      with no difference between the 6-MP and the 6-MP plus MTX groups. CONCLUSIONS: 
      Single bolus doses of MTX increase the concentration of MMPRP in rats given daily 
      s.c. doses of 6-MP, with no effect on 6-TGN concentration or TPMT activity.
FAU - Giverhaug, T
AU  - Giverhaug T
AD  - Department of Pharmacology, University of Tromso, Norway.
FAU - Fuskevaag, O M
AU  - Fuskevaag OM
FAU - Aarbakke, J
AU  - Aarbakke J
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - Germany
TA  - Cancer Chemother Pharmacol
JT  - Cancer chemotherapy and pharmacology
JID - 7806519
RN  - 0 (Guanine Nucleotides)
RN  - 0 (Nucleic Acid Synthesis Inhibitors)
RN  - 0 (Thionucleotides)
RN  - 15867-02-4 (6-thioguanylic acid)
RN  - 342-69-8 (Methylthioinosine)
RN  - E7WED276I5 (Mercaptopurine)
RN  - EC 2.1.1.- (Methyltransferases)
RN  - EC 2.1.1.67 (thiopurine methyltransferase)
RN  - YL5FZ2Y5U1 (Methotrexate)
SB  - IM
MH  - Animals
MH  - Erythrocytes/drug effects/*metabolism
MH  - Guanine Nucleotides/analysis/metabolism
MH  - Male
MH  - Mercaptopurine/*metabolism/pharmacology
MH  - Methotrexate/*pharmacology
MH  - Methylthioinosine/analysis/metabolism
MH  - Methyltransferases/metabolism
MH  - Nucleic Acid Synthesis Inhibitors/*pharmacology
MH  - Rats
MH  - Rats, Wistar
MH  - Thionucleotides/analysis/metabolism
EDAT- 1997/01/01 00:00
MHDA- 1997/01/01 00:01
CRDT- 1997/01/01 00:00
PHST- 1997/01/01 00:00 [pubmed]
PHST- 1997/01/01 00:01 [medline]
PHST- 1997/01/01 00:00 [entrez]
AID - 10.1007/s002800050672 [doi]
PST - ppublish
SO  - Cancer Chemother Pharmacol. 1997;40(4):367-70. doi: 10.1007/s002800050672.