PMID- 9227349
OWN - NLM
STAT- MEDLINE
DCOM- 19970801
LR  - 20220227
IS  - 0022-3417 (Print)
IS  - 0022-3417 (Linking)
VI  - 182
IP  - 1
DP  - 1997 May
TI  - The role of monocyte chemoattractant protein-1 (MCP-1) in the pathogenesis of 
      collagen-induced arthritis in rats.
PG  - 106-14
AB  - Collagen-induced arthritis was produced in rats by intradermal immunization with 
      type II collagen and the expression and production of monocyte chemoattractant 
      protein-1 (MCP-1) were examined by immunohistochemistry, enzyme-linked 
      immunosorbent assay (ELISA), and Northern blot analysis. Two to three weeks after 
      the immunization, the hindfeet showed swelling and redness, followed by the 
      development of severe arthritis, particularly in the ankle joints. During this 
      period, prominent infiltration of neutrophils and macrophages was observed. 
      Sandwich ELISA and Northern blot analysis revealed that MCP-1 concentrations in 
      the joint lavages and MCP-1 mRNA levels in the joint tissues both peaked at 2 
      weeks after the immunization. By immunohistochemistry, various types of cells, 
      particularly neutrophils, macrophages, synovial cells, and vascular endothelial 
      cells, stained positively for MCP-1. Finally, injection of a neutralizing 
      monoclonal antibody against rat MCP-1 significantly decreased the number of 
      exudate macrophages in the lesions and reduced the ankle swelling by about 30 per 
      cent compared with controls. These results suggest that MCP-1 plays a critical 
      role in this model in the recruitment of monocytes and in the development of 
      arthritis.
FAU - Ogata, H
AU  - Ogata H
AD  - Second Department of Pathology, Kumamoto University School of Medicine, Japan.
FAU - Takeya, M
AU  - Takeya M
FAU - Yoshimura, T
AU  - Yoshimura T
FAU - Takagi, K
AU  - Takagi K
FAU - Takahashi, K
AU  - Takahashi K
LA  - eng
PT  - Journal Article
PL  - England
TA  - J Pathol
JT  - The Journal of pathology
JID - 0204634
RN  - 0 (Antibodies, Monoclonal)
RN  - 0 (Chemokine CCL2)
RN  - 0 (RNA, Messenger)
RN  - 9007-34-5 (Collagen)
SB  - IM
MH  - Animals
MH  - Antibodies, Monoclonal/therapeutic use
MH  - Arthritis, Rheumatoid/etiology/*metabolism/pathology
MH  - Chemokine CCL2/genetics/immunology/*physiology
MH  - Collagen
MH  - Disease Models, Animal
MH  - Female
MH  - Immunoenzyme Techniques
MH  - RNA, Messenger/genetics
MH  - Rats
MH  - Rats, Inbred Strains
MH  - Synovial Membrane/metabolism/pathology
EDAT- 1997/05/01 00:00
MHDA- 2000/06/20 09:00
CRDT- 1997/05/01 00:00
PHST- 1997/05/01 00:00 [pubmed]
PHST- 2000/06/20 09:00 [medline]
PHST- 1997/05/01 00:00 [entrez]
AID - 10.1002/(SICI)1096-9896(199705)182:1<106::AID-PATH816>3.0.CO;2-A [pii]
AID - 10.1002/(SICI)1096-9896(199705)182:1<106::AID-PATH816>3.0.CO;2-A [doi]
PST - ppublish
SO  - J Pathol. 1997 May;182(1):106-14. doi: 
      10.1002/(SICI)1096-9896(199705)182:1<106::AID-PATH816>3.0.CO;2-A.