PMID- 9227794
OWN - NLM
STAT- MEDLINE
DCOM- 19970821
LR  - 20171006
IS  - 0386-300X (Print)
IS  - 0386-300X (Linking)
VI  - 51
IP  - 3
DP  - 1997 Jun
TI  - Gene transfection by cationic liposomes: comparison of the transfection 
      efficiency of liposomes prepared from various positively charged lipids.
PG  - 149-54
AB  - We compared the transfection efficiency of four types of positively charged 
      liposomes composed of (i) N-(alpha-trimethylammonioacetyl)-didodecyl-D-glutamate 
      chloride (TMAG), dilauroylphosphatidylcholine (DLPC), and 
      dioleoylphosphatidylethanolamine (DOPE) (1:2:2 molar ratio); (ii) 3 beta [N-(N', 
      N'-dimethylaminoethane)-carbamoyl] cholesterol (DC-Chol) and DOPE (3:2 molar 
      ratio); (iii) dimethyldioctadecylammonium bromide (DDAB) and DOPE (1:2.2 molar 
      ratio); (iv) N-[1-(2,3-dioleyloxy) propyl]-N,N,N-trimethylammonium chloride 
      (DOTMA) and DOPE (1:1, w/w; lipofectin). Luciferase gene was used as a reporter 
      gene. Among the cationic lipsomes used, the liposomes composed of TMAG, DOPE and 
      DLPC showed a much higher efficiency of plasmid DNA entrapment than the other 
      cationic liposomes tested. In the absence of serum, the cationic multilamellar 
      vesicles (MLV) and small unilamellar vesicles (SUV) composed of TMAG, DOPE and 
      DLPC gave highly efficient transfection. On the other hand, MLV, 
      dehydration-rehydration vesicles (DRV), and SUV liposomes prepared with the 
      mixtures of DC-Chol and DOPE showed similar levels of transfection efficiency. 
      However, the cationic liposomes composed of DDAB and DOPE showed inferior 
      efficiency, whether in the form of DRV, SUV or MLV. The transfection efficiency 
      of lipofectin was also low. In the presence of serum, on the other hand, a 
      considerable (about 30-50%) amount of transfection activity was still observed at 
      10% fetal calf serum in the cationic MLV and SUV composed of TMAG, DOPE and DLPC. 
      Cationic MLV, composed of TMAG, DOPE and DLPC, Cationic MLV, composed of TMAG, 
      DOPE and DLPC, can transfect plasmid DNA, not only in the adherent cell lines but 
      also, in the suspension cell lines. These findings indicate that the transfection 
      efficiency of cationic liposomes is affected by the lipid composition, the type 
      of liposome, or the presence or absence of serum. They also indicate that the 
      cationic liposomes containing TMAG, DOPE and DLPC are efficient vectors for gene 
      transfer into cells.
FAU - Zhao, D D
AU  - Zhao DD
AD  - Department of Cell Chemistry, Okayama University Medical School, Japan.
FAU - Watarai, S
AU  - Watarai S
FAU - Lee, J T
AU  - Lee JT
FAU - Kouchi, S
AU  - Kouchi S
FAU - Ohmori, H
AU  - Ohmori H
FAU - Yasuda, T
AU  - Yasuda T
LA  - eng
PT  - Comparative Study
PT  - Journal Article
PL  - Japan
TA  - Acta Med Okayama
JT  - Acta medica Okayama
JID - 0417611
RN  - 0 (Cations)
RN  - 0 (Glutamates)
RN  - 0 (Lipids)
RN  - 0 (Liposomes)
RN  - 0 (Phosphatidylethanolamines)
RN  - 0 (Quaternary Ammonium Compounds)
RN  - 104162-48-3 (N-(1-(2,3-dioleyloxy)propyl)-N,N,N-trimethylammonium)
RN  - 131897-06-8 (didodecyl N-(alpha-trimethylammonioacetyl)glutamate)
RN  - 137056-72-5 (3-(N-(N',N'-dimethylaminoethane)carbamoyl)cholesterol)
RN  - 2462-63-7 (dioleoyl phosphatidylethanolamine)
RN  - 251IW5I21C (dimethyldioctadecylammonium)
RN  - 9007-49-2 (DNA)
RN  - 97C5T2UQ7J (Cholesterol)
SB  - IM
MH  - Animals
MH  - COS Cells
MH  - Cations/*pharmacology
MH  - Cats
MH  - Cattle
MH  - Cell Line
MH  - Cholesterol/analogs & derivatives/pharmacology
MH  - DNA/drug effects/metabolism
MH  - Glutamates/pharmacology
MH  - Humans
MH  - Kidney/cytology
MH  - Lipids/chemistry/genetics/*pharmacology
MH  - Liposomes/chemical synthesis/chemistry/*pharmacology
MH  - Mice
MH  - Phosphatidylethanolamines/pharmacology
MH  - Quaternary Ammonium Compounds/pharmacology
MH  - Sheep
MH  - Transfection/*drug effects
EDAT- 1997/06/01 00:00
MHDA- 1997/06/01 00:01
CRDT- 1997/06/01 00:00
PHST- 1997/06/01 00:00 [pubmed]
PHST- 1997/06/01 00:01 [medline]
PHST- 1997/06/01 00:00 [entrez]
AID - 10.18926/AMO/30793 [doi]
PST - ppublish
SO  - Acta Med Okayama. 1997 Jun;51(3):149-54. doi: 10.18926/AMO/30793.