PMID- 9228712
OWN - NLM
STAT- MEDLINE
DCOM- 19970912
LR  - 20190819
IS  - 0042-9007 (Print)
IS  - 0042-9007 (Linking)
VI  - 72
IP  - 4
DP  - 1997
TI  - Prolonged holding of whole blood at 22 degrees C does not increase activation in 
      platelet concentrates.
PG  - 225-8
AB  - OBJECTIVES: Platelets prepared after holding of whole blood overnight at 22 
      degrees C have a well-preserved metabolism. However, the possibility that such 
      prolonged incubation with active granulocytes may increase platelet activation 
      has not been fully tested. METHODS: We investigated this possibility by flow 
      cytometric analysis of membrane glycoproteins (GPs) Ib and IIb/IIIa and the 
      activation markers CD62P and CD63 in platelet concentrates (PCs) prepared from 
      whole blood that was held for either 6 h without cooling plates (n = 20) or for 
      24 h on cooling plates of 1,4-butanediol (n = 20). PCs were prepared by the 
      platelet-rich plasma method and analyzed on the second storage day. RESULTS: 
      Platelet yield and aggregation response to ristocetin, collagen and epinephrine + 
      ADP were similar in both types of PCs, as was the mean fluorescence intensity for 
      GPs Ib and IIb/IIIa. PCs prepared by the overnight-hold method did not differ 
      from those obtained 6 h after collection in the percentage of platelets 
      expressing CD62P (12.3 +/- 6.2% vs. 14.1 +/- 4.0%; p > 0.1) or CD63 (9.8 +/- 6.4% 
      vs. 8.8 +/- 3.6%; p > 0.1). CONCLUSION: Prolonged holding of whole blood at 22 
      degrees C prior to component preparation does not increase the level of platelet 
      activation.
FAU - Sanz, C
AU  - Sanz C
AD  - Service of Hemotherapy and Hemostasis, Hospital Clinico, Barcelona, Spain.
FAU - Pereira, A
AU  - Pereira A
FAU - Faundez, A I
AU  - Faundez AI
FAU - Ordinas, A
AU  - Ordinas A
LA  - eng
PT  - Comparative Study
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - England
TA  - Vox Sang
JT  - Vox sanguinis
JID - 0413606
RN  - 0 (Antigens, CD)
RN  - 0 (Biomarkers)
RN  - 0 (CD63 protein, human)
RN  - 0 (P-Selectin)
RN  - 0 (Platelet Membrane Glycoproteins)
RN  - 0 (Tetraspanin 30)
RN  - 1404-55-3 (Ristocetin)
RN  - 61D2G4IYVH (Adenosine Diphosphate)
RN  - 9007-34-5 (Collagen)
RN  - YKH834O4BH (Epinephrine)
SB  - IM
MH  - Adenosine Diphosphate/pharmacology
MH  - Antigens, CD/analysis
MH  - Biomarkers
MH  - Blood Preservation/*methods
MH  - Collagen/pharmacology
MH  - Epinephrine/pharmacology
MH  - Flow Cytometry
MH  - Granulocytes/physiology
MH  - Humans
MH  - P-Selectin/blood
MH  - *Platelet Activation
MH  - Platelet Aggregation/drug effects
MH  - Platelet Membrane Glycoproteins/analysis
MH  - Ristocetin/pharmacology
MH  - Temperature
MH  - Tetraspanin 30
MH  - Time Factors
EDAT- 1997/01/01 00:00
MHDA- 1997/01/01 00:01
CRDT- 1997/01/01 00:00
PHST- 1997/01/01 00:00 [pubmed]
PHST- 1997/01/01 00:01 [medline]
PHST- 1997/01/01 00:00 [entrez]
AID - 10.1046/j.1423-0410.1997.7240225.x [doi]
PST - ppublish
SO  - Vox Sang. 1997;72(4):225-8. doi: 10.1046/j.1423-0410.1997.7240225.x.