PMID- 9231982
OWN - NLM
STAT- MEDLINE
DCOM- 19970905
LR  - 20200407
IS  - 0378-1135 (Print)
IS  - 1873-2542 (Electronic)
IS  - 0378-1135 (Linking)
VI  - 57
IP  - 1
DP  - 1997 Jun 30
TI  - Porcine reproductive and respiratory syndrome virus: routes of excretion.
PG  - 69-81
AB  - This study was conducted to delineate potential sites of exit and duration of 
      shedding of porcine reproductive and respiratory syndrome virus (PRRSV). Two 
      experiments of 6 pigs each were conducted. Pigs were farrowed in isolation, 
      weaned at 7 days of age, and housed in individual HEPA filtered isolation 
      chambers. In each experiment, 3 pigs served as controls and 3 were inoculated 
      intranasally with PRRSV (ATCC VR-2402) at 3 weeks of age. In a first experiment, 
      on days 7, 14, 21, 28, 35, and 42 post-inoculation (p.i.), pigs were anesthetized 
      and intubated. The following samples were collected: serum, saliva, conjunctival 
      swabs, urine by cystocentesis, and feces. Upon recovery from anesthesia, the 
      endotracheal tube was removed, rinsed, and the rinse retained. In the second 
      experiment, the sampling schedule was expanded and serum, saliva, and 
      oropharyngeal samples were collected from day 55 to day 124 p.i. at 14 day 
      intervals. Virus was isolated in porcine alveolar macrophages up to day 14 from 
      urine, day 21 from serum, day 35 from endotracheal tube rinse, day 42 from 
      saliva, and day 84 from oropharyngeal samples. No virus was recovered from 
      conjunctival swabs, fecal samples, or negative control samples. This is the first 
      report of isolation of PRRSV from saliva. Virus-contaminated saliva, especially 
      when considered in the context of social dominance behavior among pigs, may plan 
      an important role in PRRSV transmission. These results support previous reports 
      of persistent infection with PRRSV with prolonged recovery of virus from tonsils 
      of swine.
FAU - Wills, R W
AU  - Wills RW
AD  - Department of Veterinary and Biomedical Sciences, University of Nebraska-Lincoln 
      68583, USA. vets078@unlvm.unl.edu
FAU - Zimmerman, J J
AU  - Zimmerman JJ
FAU - Yoon, K J
AU  - Yoon KJ
FAU - Swenson, S L
AU  - Swenson SL
FAU - Hoffman, L J
AU  - Hoffman LJ
FAU - McGinley, M J
AU  - McGinley MJ
FAU - Hill, H T
AU  - Hill HT
FAU - Platt, K B
AU  - Platt KB
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - Netherlands
TA  - Vet Microbiol
JT  - Veterinary microbiology
JID - 7705469
SB  - IM
MH  - Animals
MH  - Female
MH  - Hemagglutination Inhibition Tests
MH  - Oropharynx/virology
MH  - Porcine Reproductive and Respiratory Syndrome/*virology
MH  - Porcine respiratory and reproductive syndrome virus/*isolation & purification
MH  - Saliva/virology
MH  - Swine
MH  - Trachea/virology
MH  - Urine/virology
MH  - Viremia/virology
PMC - PMC7117148
EDAT- 1997/06/30 00:00
MHDA- 1997/06/30 00:01
PMCR- 1998/01/05
CRDT- 1997/06/30 00:00
PHST- 1997/06/30 00:00 [pubmed]
PHST- 1997/06/30 00:01 [medline]
PHST- 1997/06/30 00:00 [entrez]
PHST- 1998/01/05 00:00 [pmc-release]
AID - S0378113597000795 [pii]
AID - S0378-1135(97)00079-5 [pii]
AID - 10.1016/s0378-1135(97)00079-5 [doi]
PST - ppublish
SO  - Vet Microbiol. 1997 Jun 30;57(1):69-81. doi: 10.1016/s0378-1135(97)00079-5.