PMID- 9232026
OWN - NLM
STAT- MEDLINE
DCOM- 19970821
LR  - 20191102
IS  - 0962-8819 (Print)
IS  - 0962-8819 (Linking)
VI  - 6
IP  - 4
DP  - 1997 Jul
TI  - Analysis of transgene integration sites in transgenic pigs by fluorescence in 
      situ hybridization.
PG  - 253-9
AB  - The production of pigs transgenic for human decay accelerating factor (hDAF) as 
      potential donors for clinical organ xenotransplantation was reported several 
      years ago. For this purpose it is required that high levels of hDAF are expressed 
      at relevant sites in transplantable organs. Currently, homozygous lines have been 
      produced as well as lines from crosses between heterozygous animals from 
      different founder lines, termed 'jigsaw' pigs. The purpose of the 'jigsaw' 
      crosses is to combine the desirable hDAF protein expression patterns found in 
      different founder lines. Initial selection of the 'jigsaw' pigs is based on the 
      inheritance of the hDAF integration sites from both lines. Litters with potential 
      homozygous transgenics and 'jigsaw' transgenics were analysed by fluorescence in 
      situ hybridization (FISH) and slot blot analysis. Results show that both slot 
      blot analysis and FISH are suitable to distinguish between pigs that are 
      heterozygous and homozygous fir hDAF. However, FISH has the advantage of 
      producing results more rapidly. For the identification of 'jigsaw' pigs FISH 
      analysis was required since slot blot analysis lacked the required accuracy. On 
      basis of these results, FISH analysis was made part of the routine screening 
      programme for hDAF transgenic pigs.
FAU - Kuipers, H W
AU  - Kuipers HW
AD  - Department of Surgery, University of Cambridge, UK.
FAU - Langford, G A
AU  - Langford GA
FAU - White, D J
AU  - White DJ
LA  - eng
PT  - Comparative Study
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - Netherlands
TA  - Transgenic Res
JT  - Transgenic research
JID - 9209120
RN  - 0 (CD55 Antigens)
RN  - 0 (DNA Probes)
RN  - 1405-69-2 (Avidin)
SB  - IM
MH  - Animals
MH  - Animals, Genetically Modified
MH  - Avidin/chemistry
MH  - CD55 Antigens/genetics/metabolism
MH  - Crosses, Genetic
MH  - DNA Probes
MH  - Endothelium/metabolism
MH  - Female
MH  - Fluorescent Antibody Technique, Direct/methods
MH  - Fluorescent Antibody Technique, Indirect
MH  - Heterozygote
MH  - Homozygote
MH  - Humans
MH  - In Situ Hybridization, Fluorescence/*methods
MH  - Male
MH  - Metaphase
MH  - Swine/*genetics
MH  - Tissue Distribution
MH  - *Transgenes
EDAT- 1997/07/01 00:00
MHDA- 1997/07/01 00:01
CRDT- 1997/07/01 00:00
PHST- 1997/07/01 00:00 [pubmed]
PHST- 1997/07/01 00:01 [medline]
PHST- 1997/07/01 00:00 [entrez]
AID - 10.1023/a:1018402510471 [doi]
PST - ppublish
SO  - Transgenic Res. 1997 Jul;6(4):253-9. doi: 10.1023/a:1018402510471.