PMID- 9237724 OWN - NLM STAT- MEDLINE DCOM- 19970825 LR - 20190512 IS - 0022-1899 (Print) IS - 0022-1899 (Linking) VI - 176 IP - 2 DP - 1997 Aug TI - A new multiantigen immunoassay for the quantification of IgG antibodies to capsular polysaccharides of Streptococcus pneumoniae. PG - 526-9 AB - A new nitrocellulose-based solid-phase multiantigen immunoassay (MAIA) for the detection of serum antibodies to Streptococcus pneumoniae capsular polysaccharides (PPSs) is presented. Evaluation with human sera showed that the MAIA test is reproducible, sensitive, and specific. It correlated well with a conventional ELISA method. The multiantigen strip system allowed quantification of antibodies against several PPS serotypes simultaneously and with a minimal amount of serum specimen. The presented solid-phase immunoassay for the quantification of anti-PPS antibodies seems to be a superior and attractive alternative to currently used ELISA tests and offers possibilities for standardization. FAU - Roth, F AU - Roth F AD - Institute of Medical Microbiology, University of Berne, Switzerland. FAU - Burkart, T AU - Burkart T FAU - Muhlemann, K AU - Muhlemann K LA - eng PT - Comparative Study PT - Journal Article PL - United States TA - J Infect Dis JT - The Journal of infectious diseases JID - 0413675 RN - 0 (Antibodies, Bacterial) RN - 0 (Antigens, Bacterial) RN - 0 (Bacterial Vaccines) RN - 0 (Immune Sera) SB - IM MH - Adult MH - Antibodies, Bacterial/*blood MH - Antigens, Bacterial MH - Bacterial Capsules/*immunology MH - Bacterial Vaccines MH - Cell Wall MH - Enzyme-Linked Immunosorbent Assay MH - Fetal Blood MH - Humans MH - Immune Sera MH - *Immunoenzyme Techniques MH - Sensitivity and Specificity MH - Streptococcus pneumoniae/*immunology EDAT- 1997/08/01 00:00 MHDA- 1997/08/01 00:01 CRDT- 1997/08/01 00:00 PHST- 1997/08/01 00:00 [pubmed] PHST- 1997/08/01 00:01 [medline] PHST- 1997/08/01 00:00 [entrez] AID - 10.1086/517281 [doi] PST - ppublish SO - J Infect Dis. 1997 Aug;176(2):526-9. doi: 10.1086/517281.