PMID- 9237761
OWN - NLM
STAT- MEDLINE
DCOM- 19970925
LR  - 20190512
IS  - 0267-8357 (Print)
IS  - 0267-8357 (Linking)
VI  - 12
IP  - 4
DP  - 1997 Jul
TI  - Detection of chromosomal alterations affecting the 1cen-1q12 region in irradiated 
      granulocytes and lymphocytes by multicolour FISH with tandem DNA probes.
PG  - 195-200
AB  - A multicolour tandem labelling fluorescence in situ hybridization (FISH) 
      procedure was used to compare the frequencies of radiation-induced chromosome 
      breakage and hyperdiploidy of chromosome 1 occurring in non-cultured granulocytes 
      and Go lymphocytes with those observed in cultured metaphase and interphase 
      lymphocytes. Whole blood, obtained from healthy male donors, was exposed in vitro 
      to 0, 100, 200, 300 and 400 cGy of ionizing radiation from a 137Cs source. 
      Aliquots containing granulocytes and Go lymphocytes from each dose were treated 
      immediately with hypotonic KCI on ice and harvested. Cells were hybridized with 
      alpha- and classical satellite probes to the 1cen-q12 region of chromosome 1 and 
      the frequencies of hyperdiploidy and breakage affecting this region were 
      determined. Elevated dose-related frequencies of breakage were detectable in both 
      lymphocytes and granulocytes immediately following radiation and decreased 
      rapidly over the first 0.25-2 h. In a second series of experiments, the 
      frequencies of hyperdiploidy and breakage for uncultured granulocytes and Go 
      lymphocytes were compared with interphase and metaphase cells following 48-51 h 
      of culture. Similar and significant dose-related increases in breakage were seen 
      for the granulocytes, Go lymphocytes, 48 h cultured interphase and metaphase 
      lymphocytes. A minor increase in hyperdiploidy was seen in the irradiated 
      cultured cells, whereas no hyperdiploid cells were detected in the non-cultured 
      cells. These results indicate that, in general, granulocytes and lymphocytes show 
      similar sensitivity to radiation-induced damage and that cell culture is not 
      required for chromosome breakage to be observed microscopically using this FISH 
      procedure.
FAU - Rupa, D S
AU  - Rupa DS
AD  - Enviromental Toxicology Graduate Program, Department of Entomology, University of 
      California, Riverside 92521, USA.
FAU - Hasegawa, L S
AU  - Hasegawa LS
FAU - Eastmond, D A
AU  - Eastmond DA
LA  - eng
PT  - Comparative Study
PT  - Journal Article
PT  - Research Support, U.S. Gov't, Non-P.H.S.
PL  - England
TA  - Mutagenesis
JT  - Mutagenesis
JID - 8707812
RN  - 0 (DNA Probes)
SB  - IM
MH  - Cells, Cultured
MH  - Centromere/genetics
MH  - *Chromosome Aberrations
MH  - Chromosome Breakage
MH  - *Chromosomes, Human, Pair 1
MH  - DNA Probes
MH  - Diploidy
MH  - Dose-Response Relationship, Radiation
MH  - Granulocytes/physiology/*radiation effects
MH  - Humans
MH  - In Situ Hybridization, Fluorescence/*methods
MH  - Interphase/genetics/radiation effects
MH  - Lymphocytes/physiology/*radiation effects
MH  - Male
MH  - Metaphase/genetics/radiation effects
MH  - Sensitivity and Specificity
MH  - Time Factors
EDAT- 1997/07/01 00:00
MHDA- 1997/07/01 00:01
CRDT- 1997/07/01 00:00
PHST- 1997/07/01 00:00 [pubmed]
PHST- 1997/07/01 00:01 [medline]
PHST- 1997/07/01 00:00 [entrez]
AID - 10.1093/mutage/12.4.195 [doi]
PST - ppublish
SO  - Mutagenesis. 1997 Jul;12(4):195-200. doi: 10.1093/mutage/12.4.195.