PMID- 9237762
OWN - NLM
STAT- MEDLINE
DCOM- 19970925
LR  - 20190512
IS  - 0267-8357 (Print)
IS  - 0267-8357 (Linking)
VI  - 12
IP  - 4
DP  - 1997 Jul
TI  - Extruded micronuclei induced by colchicine or acrylamide contain mostly lagging 
      chromosomes identified in paintbrush smears by minor and major mouse DNA probes.
PG  - 201-7
AB  - In the mouse bone marrow micronucleus assay, it was studied whether micronuclei 
      (MN) could be expelled from polychromatic erythrocytes (PCE) in a similar way to 
      the main nucleus. To avoid the disrupting centrifugation step of the conventional 
      bone marrow preparation procedure, the paintbrush technique was used in the 
      present experiments. With May-Grunwald-Giemsa staining of paintbrush slides, 5 % 
      of the colchicine (COL)-induced MN were found attached to the outside membranes 
      of PCE and were regarded as extruded. Of the acrylamide (AA)-induced MN, 22% were 
      extruded. After fluorescence in situ hybridization (FISH) of a total of 300 MN 
      per chemical treatment with the mouse minor and major satellite DNA probes, 9.7% 
      MN were extruded in the COL group and 8.3% MN were extruded in the AA group. FISH 
      showed that 76% of the retained COL-induced MN were signal-positive, indicating 
      that they contained entire chromosomes. With AA, 29% minor-positive and 28.3 % 
      major-positive retained MN were found, confirming its known clastogenicity. 
      However, the observed frequency of signal-positive MN (1.7 MNPCE(pos)/ 1000 PCE) 
      in the AA group was about three times higher than in the control (0.5 
      MNPCE(pos)/1000 PCE) which indicates that AA has aneugenic potential. FISH 
      analysis of the extruded MN showed 72-100% major as well as minor signals. It is 
      concluded that expelled MN contain mostly entire chromosomes.
FAU - Schriever-Schwemmer, G
AU  - Schriever-Schwemmer G
AD  - Ludwigs-Maximillians-Universitat Munchen, Tierarzliche Fakultat, Lehrstuhl fur 
      Allgemeine Pathologie und Neuropathologie, Germany.
FAU - Kliesch, U
AU  - Kliesch U
FAU - Adler, I D
AU  - Adler ID
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - England
TA  - Mutagenesis
JT  - Mutagenesis
JID - 8707812
RN  - 0 (Acrylamides)
RN  - 0 (DNA Probes)
RN  - 20R035KLCI (Acrylamide)
RN  - SML2Y3J35T (Colchicine)
SB  - IM
MH  - Acrylamide
MH  - Acrylamides/*toxicity
MH  - Animals
MH  - Colchicine/*toxicity
MH  - *DNA Probes
MH  - Erythrocytes/drug effects
MH  - Genetic Techniques
MH  - In Situ Hybridization, Fluorescence/methods
MH  - Male
MH  - Mice
MH  - Mice, Inbred C3H
MH  - Mice, Inbred Strains
MH  - Micronuclei, Chromosome-Defective/*drug effects
MH  - Micronucleus Tests/*methods
MH  - Staining and Labeling/methods
EDAT- 1997/07/01 00:00
MHDA- 1997/07/01 00:01
CRDT- 1997/07/01 00:00
PHST- 1997/07/01 00:00 [pubmed]
PHST- 1997/07/01 00:01 [medline]
PHST- 1997/07/01 00:00 [entrez]
AID - 10.1093/mutage/12.4.201 [doi]
PST - ppublish
SO  - Mutagenesis. 1997 Jul;12(4):201-7. doi: 10.1093/mutage/12.4.201.