PMID- 9237815
OWN - NLM
STAT- MEDLINE
DCOM- 19971010
LR  - 20061115
IS  - 1043-4666 (Print)
IS  - 1043-4666 (Linking)
VI  - 9
IP  - 7
DP  - 1997 Jul
TI  - Migration responses of human monocytic cell lines to alpha- and beta-chemokines.
PG  - 521-8
AB  - The beta-chemokines monocyte chemotactic protein 1 (MCP-1), macrophage 
      inflammatory protein 1 alpha (MIP-1alpha), MIP-1beta and regulated on activation, 
      normal T cells, expressed and secreted (RANTES) induced the in vitro migration of 
      the monocytic cell line MonoMac-6. MCP-1 exhibits the most potent chemotactic 
      effect on this cell line while MIP-1alpha, RANTES and to a lesser extent 
      MIP-1beta were more moderate inducers of cell migration. MonoMac-6 migration in 
      response to chemokines was shown to be a chemotactic and not a chemokinetic 
      response, which was inhibited by pertussis and cholera toxins suggesting a role 
      for G proteins in chemokine receptor-mediated signalling in these cells; 
      chemotaxis of MonoMac-6 cells in response to MCP-1 was abrogated by the addition 
      of anti-MCP-1 antibody. The response of MonoMac-6 cells to the alpha-chemokines 
      IL-8, IP-10, growth-related peptide (Gro) alpha and MIP-2beta was substantially 
      weaker than to the beta-chemokines. MCP-1 caused an alteration in cellular 
      morphology by increasing ruffling at the cell membrane and the number of cells 
      exhibiting extended pseudopodia. The chemotactic response of MonoMac-6 cells to 
      beta-chemokines was compared with less well-differentiated myelomonocytic cell 
      lines. THP-1 showed a similar, but weaker response to the beta-chemokines while 
      both HL60 and U937 failed to respond to any member of this subfamily when tested 
      under the same conditions. These results suggest that the differentiation status 
      of cells of monocytic lineage may affect their response to beta-chemokines.
FAU - Cross, A K
AU  - Cross AK
AD  - Institute for Cancer Studies, University of Sheffield Medical School, UK.
FAU - Richardson, V
AU  - Richardson V
FAU - Ali, S A
AU  - Ali SA
FAU - Palmer, I
AU  - Palmer I
FAU - Taub, D D
AU  - Taub DD
FAU - Rees, R C
AU  - Rees RC
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - England
TA  - Cytokine
JT  - Cytokine
JID - 9005353
RN  - 0 (Chemokine CCL2)
RN  - 0 (Chemokines)
RN  - 0 (Chemotactic Factors)
SB  - IM
MH  - Cell Line
MH  - Chemokine CCL2/pharmacology
MH  - Chemokines/*pharmacology
MH  - Chemotactic Factors/*pharmacology
MH  - Chemotaxis
MH  - *Chemotaxis, Leukocyte
MH  - HL-60 Cells
MH  - Humans
MH  - Monocytes/*cytology
MH  - Tumor Cells, Cultured
EDAT- 1997/07/01 00:00
MHDA- 1997/07/01 00:01
CRDT- 1997/07/01 00:00
PHST- 1997/07/01 00:00 [pubmed]
PHST- 1997/07/01 00:01 [medline]
PHST- 1997/07/01 00:00 [entrez]
AID - S1043-4666(96)90196-9 [pii]
AID - 10.1006/cyto.1996.0196 [doi]
PST - ppublish
SO  - Cytokine. 1997 Jul;9(7):521-8. doi: 10.1006/cyto.1996.0196.