PMID- 9238650
OWN - NLM
STAT- MEDLINE
DCOM- 19970911
LR  - 20190512
IS  - 1360-9947 (Print)
IS  - 1360-9947 (Linking)
VI  - 2
IP  - 1
DP  - 1996 Jan
TI  - The receptor encoded by the human C-MET oncogene is expressed in testicular 
      tissue and on human spermatozoa.
PG  - 2-8
AB  - Because of its distinctive ability to act as a mitogen, a mitogen and a 
      morphogen, hepatocyte growth factor/scatter factor (HGF/SF) has all the 
      characteristics of a molecule able to function in regulatory networks of 
      motility, such as the spermatogenic epithelium, and this through binding of its 
      receptor p190MET (C-MET). In this study we report the expression of C-MET in the 
      human seminiferous epithelium and on spermatozoa from men being treated for 
      infertility and sperm donors. The presence of C-MET was demonstrated by 
      immunochemistry on the cell membrane of spermatogonia, spermatocytes, spermatids 
      and on spermatozoa, whereas Sertoli cells and Leydig cells did not show 
      expression. Comparison of C-MET expression on spermatozoa of the 90% Percoll 
      layer of subfertile patients and donors revealed clearly two distinct groups 
      (unpaired t-test, P < 0.001), whereas comparison of C-MET expression on 
      spermatozoa in the 47% Percoll layer was not significantly different between 
      patients and donors. In addition, there was a significant inverse correlation 
      between sperm concentration and the C-MET expression of spermatozoa in the 90% 
      Percoll layer (r = -0.80, 95% confidence interval, -0.92 to -0.55; P < 0.0001), 
      but not with the C-MET expression of spermatozoa in the 47% Percoll layer. In 
      conclusion, the presence of C-MET was demonstrated in the seminiferous epithelium 
      and on mature and immature spermatozoa, indicating a role for this growth factor 
      receptor in the differentiation and/or migration that occurs during human 
      spermatogenesis.
FAU - Depuydt, C E
AU  - Depuydt CE
AD  - Department of Internal Medicine, University Hospital, Ghent, Belgium.
FAU - Zalata, A
AU  - Zalata A
FAU - de Potter, C R
AU  - de Potter CR
FAU - van Emmelo, J
AU  - van Emmelo J
FAU - Comhaire, F H
AU  - Comhaire FH
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - England
TA  - Mol Hum Reprod
JT  - Molecular human reproduction
JID - 9513710
RN  - 0 (Proto-Oncogene Proteins)
RN  - 67256-21-7 (Hepatocyte Growth Factor)
RN  - EC 2.7.10.1 (Proto-Oncogene Proteins c-met)
RN  - EC 2.7.10.1 (Receptor Protein-Tyrosine Kinases)
SB  - IM
MH  - Blotting, Western
MH  - Cell Line
MH  - Flow Cytometry
MH  - Fluorescent Antibody Technique, Indirect
MH  - Hepatocyte Growth Factor/*metabolism
MH  - Humans
MH  - Immunohistochemistry
MH  - Infertility, Male
MH  - Insemination, Artificial
MH  - Male
MH  - Proto-Oncogene Proteins/*metabolism
MH  - Proto-Oncogene Proteins c-met
MH  - Receptor Protein-Tyrosine Kinases/*metabolism
MH  - Seminiferous Epithelium/metabolism
MH  - Spermatogenesis
MH  - Spermatozoa/*metabolism
MH  - Statistics, Nonparametric
MH  - Testis/*metabolism
MH  - Tissue Donors
EDAT- 1996/01/01 00:00
MHDA- 1996/01/01 00:01
CRDT- 1996/01/01 00:00
PHST- 1996/01/01 00:00 [pubmed]
PHST- 1996/01/01 00:01 [medline]
PHST- 1996/01/01 00:00 [entrez]
AID - 10.1093/molehr/2.1.2 [doi]
PST - ppublish
SO  - Mol Hum Reprod. 1996 Jan;2(1):2-8. doi: 10.1093/molehr/2.1.2.