PMID- 9238669
OWN - NLM
STAT- MEDLINE
DCOM- 19970917
LR  - 20190512
IS  - 1360-9947 (Print)
IS  - 1360-9947 (Linking)
VI  - 2
IP  - 2
DP  - 1996 Feb
TI  - Nuclear retinoid receptor expression in normal human endometrium throughout the 
      menstrual cycle.
PG  - 123-9
AB  - Previous work has shown that retinoic acid receptors (RARs) and retinoid X 
      receptors (RXRs) are expressed in human endometrial epithelial and stromal cells. 
      These nuclear receptors mediate the biological effects of retinoic acid, a 
      vitamin A derivative which may have an important, though poorly characterized 
      role in the functional differentiation of secretory epithelia. The aim of this 
      study was to find out whether the expression of RAR and RXR mRNA in endometrial 
      epithelial and stromal cells varies in relation to the menstrual cycle. The 
      expression of RARs and RXRs was investigated by Northern blotting and, for 
      stromal cells, there were no differences in expression of RAR-alpha, RAR-beta, 
      RAR-gamma and RXR-alpha between the proliferative and secretory phases of the 
      menstrual cycle. Similarly, for epithelial tissue, there were no significant 
      differences between the proliferative and secretory phases with respect to the 
      expression of RAR-alpha, RAR-gamma and RXR-alpha. However, RAR-beta was expressed 
      at a 1.7-fold higher level in epithelial samples from the proliferative phase 
      compared to the secretory phase. Overall, the levels of expression of RAR-alpha, 
      RAR-beta and RAR-gamma were 1.7- to 4-fold higher in stromal cells compared to 
      epithelial cells whereas RXR-alpha was expressed at a similar level in both cell 
      types. We have previously suggested that retinoic acid has a role in endometrial 
      differentiation or function which may be reflected by cyclical changes in 
      intracellular retinoic acid levels. These data indicate that RARs and RXRs are 
      expressed at a similar level throughout the menstrual cycle, with the possible 
      exception of RAR-beta, implying that any menstrual cycle-related function of RARs 
      in controlled by ligand availability rather than by changes in expression of the 
      receptors.
FAU - Kumarendran, M K
AU  - Kumarendran MK
AD  - Department of Obstetrics and Gynaecology, Medical School, University of 
      Newcastle, Newcastle upon Tyne, UK.
FAU - Loughney, A D
AU  - Loughney AD
FAU - Prentice, A
AU  - Prentice A
FAU - Thomas, E J
AU  - Thomas EJ
FAU - Redfern, C P
AU  - Redfern CP
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - England
TA  - Mol Hum Reprod
JT  - Molecular human reproduction
JID - 9513710
RN  - 0 (DNA, Complementary)
RN  - 0 (Nuclear Proteins)
RN  - 0 (Receptors, Retinoic Acid)
RN  - 0 (Retinoid X Receptors)
RN  - 0 (Transcription Factors)
RN  - 63231-63-0 (RNA)
SB  - IM
MH  - Blotting, Northern
MH  - DNA, Complementary
MH  - Endometrium/cytology/*metabolism
MH  - Epithelium/metabolism
MH  - Female
MH  - Gene Expression
MH  - Humans
MH  - Menstrual Cycle/*physiology
MH  - Nuclear Proteins/*biosynthesis/genetics
MH  - RNA/isolation & purification
MH  - Receptors, Retinoic Acid/*biosynthesis/genetics
MH  - Retinoid X Receptors
MH  - Statistics, Nonparametric
MH  - Stromal Cells/metabolism
MH  - Transcription Factors/*biosynthesis/genetics
EDAT- 1996/02/01 00:00
MHDA- 1996/02/01 00:01
CRDT- 1996/02/01 00:00
PHST- 1996/02/01 00:00 [pubmed]
PHST- 1996/02/01 00:01 [medline]
PHST- 1996/02/01 00:00 [entrez]
AID - 10.1093/molehr/2.2.123 [doi]
PST - ppublish
SO  - Mol Hum Reprod. 1996 Feb;2(2):123-9. doi: 10.1093/molehr/2.2.123.