PMID- 9242212
OWN - NLM
STAT- MEDLINE
DCOM- 19970818
LR  - 20190816
IS  - 0165-4608 (Print)
IS  - 0165-4608 (Linking)
VI  - 97
IP  - 1
DP  - 1997 Aug
TI  - Multiple chromosomal mechanisms generate an EWS/FLI1 or an EWS/ERG fusion gene in 
      Ewing tumors.
PG  - 12-9
AB  - The t(11;22)(q24;q12) translocation is found in about 85% of Ewing tumors and 
      leads in all cases to an EWS/FLI1 fusion gene. In a few instances, complex 
      translocations, involving chromosomes 11, 22 and a third chromosome or other 
      variant translocations not involving chromosome 11 also have been reported. These 
      rearrangements generate an active fusion gene between the EWS gene and either the 
      human FLI1 gene or other members of the ETS gene family: the ERG gene localized 
      in band 21q22.2, the ETV1 gene localized in band 7p22, or the E1AF gene localized 
      in band 17q12. Using fluorescence in situ hybridization (FISH) on interphase 
      nuclei or metaphases, we report here the characterization of particular 
      karyotypes in Ewing tumors, namely a complex t(2;11;22) translocation, a variant 
      t(12;22) translocation, and one Ewing tumor without specific rearrangements but 
      with an EWS/ERG fusion gene demonstrated by molecular analysis. These molecular 
      cytogenetic methods allowed us (1) to precisely localize the genomic breakpoints 
      within-EWSR1 and EWSR2 and to identify the chromosome carrying the fusion gene; 
      (2) to determine the nature of events generating the fusion genes; (3) to 
      demonstrate that some variant translocations represent masked complex 
      translocations; and (4) to show that the generation of an EWS/ERG fusion gene 
      cannot be obtained through a simple balanced translocation.
FAU - Desmaze, C
AU  - Desmaze C
AD  - Laboratory of Tumor Genetics, Curie Institute, Paris, France.
FAU - Brizard, F
AU  - Brizard F
FAU - Turc-Carel, C
AU  - Turc-Carel C
FAU - Melot, T
AU  - Melot T
FAU - Delattre, O
AU  - Delattre O
FAU - Thomas, G
AU  - Thomas G
FAU - Aurias, A
AU  - Aurias A
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - Cancer Genet Cytogenet
JT  - Cancer genetics and cytogenetics
JID - 7909240
RN  - 0 (DNA-Binding Proteins)
RN  - 0 (Heterogeneous-Nuclear Ribonucleoproteins)
RN  - 0 (Oncogene Proteins, Fusion)
RN  - 0 (Proto-Oncogene Protein c-fli-1)
RN  - 0 (Proto-Oncogene Proteins)
RN  - 0 (RNA-Binding Protein EWS)
RN  - 0 (Ribonucleoproteins)
RN  - 0 (Trans-Activators)
SB  - IM
MH  - *Chromosome Aberrations
MH  - Chromosomes, Human, Pair 11/genetics
MH  - Chromosomes, Human, Pair 12/genetics
MH  - Chromosomes, Human, Pair 2/genetics
MH  - Chromosomes, Human, Pair 21/genetics
MH  - Chromosomes, Human, Pair 22/genetics
MH  - Cosmids
MH  - DNA-Binding Proteins/*genetics
MH  - Heterogeneous-Nuclear Ribonucleoproteins
MH  - Humans
MH  - In Situ Hybridization, Fluorescence
MH  - Karyotyping
MH  - Oncogene Proteins, Fusion/*genetics
MH  - Proto-Oncogene Protein c-fli-1
MH  - *Proto-Oncogene Proteins
MH  - RNA-Binding Protein EWS
MH  - Ribonucleoproteins/*genetics
MH  - Sarcoma, Ewing/*genetics
MH  - Trans-Activators/*genetics
MH  - *Translocation, Genetic
MH  - Tumor Cells, Cultured
EDAT- 1997/08/01 00:00
MHDA- 1997/08/01 00:01
CRDT- 1997/08/01 00:00
PHST- 1997/08/01 00:00 [pubmed]
PHST- 1997/08/01 00:01 [medline]
PHST- 1997/08/01 00:00 [entrez]
AID - S0165460896003263 [pii]
AID - 10.1016/s0165-4608(96)00326-3 [doi]
PST - ppublish
SO  - Cancer Genet Cytogenet. 1997 Aug;97(1):12-9. doi: 10.1016/s0165-4608(96)00326-3.