PMID- 9242216
OWN - NLM
STAT- MEDLINE
DCOM- 19970818
LR  - 20190816
IS  - 0165-4608 (Print)
IS  - 0165-4608 (Linking)
VI  - 97
IP  - 1
DP  - 1997 Aug
TI  - Comparison of DNA flow cytometry and fluorescence in situ hybridization with a 
      set of 10 chromosome-specific DNA probes in four head and neck carcinomas.
PG  - 35-8
AB  - Four squamous cell carcinomas of the oral cavity were simultaneously analysed by 
      DNA flow cytometry (FCM) and fluorescence in situ hybridization (FISH) with 
      alpha-telomeric DNA probes specific for chromosomes 1, 2, 3, 6, 7, 8, 10, 12, 17, 
      and X. Nuclei with 2 hybridization signals for most chromosomes were in agreement 
      with flow cytometric DNA content in a diploid tumor, but a monosomy 8 for half of 
      the cells indicated a tumor clone with an aberrant chromosome 8. Aberrations of 
      virtually all chromosomes were found in 3 aneuploid tumors. Comparing the 
      relative numbers of disomal and aneusomal nuclei with the corresponding 
      proportions of DNA diploid and aneuploid sample cells provided evidence that the 
      flow cytometrically diploid cell population of an aneuploid tumor consisted of 
      both karyotypically normal and abnormal cells. Although there was a high degree 
      of concordance between aberrant DNA content and magnitude of chromosomal 
      aberration, discrepancies between both methods suggested a notable clonal 
      heterogeneity in aneuploid tumor cell populations. These results demonstrate that 
      a simultaneous application of DNA flow cytometry contributes to broaden the 
      utility of interphase cytogenetics.
FAU - Hemmer, J
AU  - Hemmer J
AD  - University of Ulm, Division of Tumor Biology, Germany.
FAU - Prinz, W
AU  - Prinz W
LA  - eng
PT  - Comparative Study
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - Cancer Genet Cytogenet
JT  - Cancer genetics and cytogenetics
JID - 7909240
RN  - 0 (DNA, Neoplasm)
SB  - IM
MH  - Aneuploidy
MH  - Carcinoma, Squamous Cell/*genetics
MH  - Chromosome Aberrations
MH  - DNA, Neoplasm/*analysis
MH  - *Flow Cytometry
MH  - Humans
MH  - *In Situ Hybridization, Fluorescence
MH  - Karyotyping
MH  - Mouth Neoplasms/*genetics
MH  - Palatal Neoplasms/genetics
MH  - Ploidies
MH  - Tongue Neoplasms/genetics
EDAT- 1997/08/01 00:00
MHDA- 1997/08/01 00:01
CRDT- 1997/08/01 00:00
PHST- 1997/08/01 00:00 [pubmed]
PHST- 1997/08/01 00:01 [medline]
PHST- 1997/08/01 00:00 [entrez]
AID - S0165460896003251 [pii]
AID - 10.1016/s0165-4608(96)00325-1 [doi]
PST - ppublish
SO  - Cancer Genet Cytogenet. 1997 Aug;97(1):35-8. doi: 10.1016/s0165-4608(96)00325-1.