PMID- 9242378
OWN - NLM
STAT- MEDLINE
DCOM- 19970821
LR  - 20121115
IS  - 0950-9232 (Print)
IS  - 0950-9232 (Linking)
VI  - 15
IP  - 4
DP  - 1997 Jul 24
TI  - Association of the Rho family small GTP-binding proteins with Rho GDP 
      dissociation inhibitor (Rho GDI) in Saccharomyces cerevisiae.
PG  - 417-22
AB  - The small GTP-binding proteins of the Rho family, consisting of the Rho, Rac, and 
      Cdc42 subfamilies, are implicated in various cell functions, such as cell shape 
      change, cell motility and cytokinesis, through reorganization of actin 
      cytoskeleton. Rho GDI is a general regulator which forms a complex with the 
      GDP-bound inactive form of the Rho family members and inhibits their activation. 
      We have purified Rho GDI from the yeast Saccharomyces cerevisiae, cloned its 
      gene, and named it RDII (Rho GD). In this study, we have further characterized 
      yeast Rho GDI. Rho GDI was found in the cytosol by immunoblot and 
      immunofluorescence microscopic analyses. Rho1p and Cdc42p were 
      co-immunoprecipitated with Rho GDI from the cytosol. This immunoprecipitated 
      Rho1p was mainly bound to GDP. In the disruption mutant of Rho GDI, which did not 
      show any apparent phenotype, both Rho1p and Cdc42p were also present in the 
      cytosol. These results indicate that yeast Rho GDI possesses properties similar 
      to those of mammalian Rho GDI, and that there is a cytosolic factor which 
      functionally substitutes for Rho GDI in yeast.
FAU - Koch, G
AU  - Koch G
AD  - Department of Molecular Biology and Biochemistry, Osaka University Medical 
      School, Suita, Japan.
FAU - Tanaka, K
AU  - Tanaka K
FAU - Masuda, T
AU  - Masuda T
FAU - Yamochi, W
AU  - Yamochi W
FAU - Nonaka, H
AU  - Nonaka H
FAU - Takai, Y
AU  - Takai Y
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - England
TA  - Oncogene
JT  - Oncogene
JID - 8711562
RN  - 0 (Fungal Proteins)
RN  - 0 (Guanine Nucleotide Dissociation Inhibitors)
RN  - 0 (Membrane Proteins)
RN  - 0 (rho-Specific Guanine Nucleotide Dissociation Inhibitors)
RN  - EC 3.6.1.- (GTP-Binding Proteins)
RN  - EC 3.6.5.2 (rhoB GTP-Binding Protein)
SB  - IM
MH  - Fungal Proteins/*physiology
MH  - GTP-Binding Proteins/analysis/*metabolism/*physiology
MH  - *Guanine Nucleotide Dissociation Inhibitors
MH  - Membrane Proteins/analysis/*metabolism
MH  - Precipitin Tests
MH  - Saccharomyces cerevisiae/*chemistry
MH  - rho-Specific Guanine Nucleotide Dissociation Inhibitors
MH  - rhoB GTP-Binding Protein
EDAT- 1997/07/24 00:00
MHDA- 1997/07/24 00:01
CRDT- 1997/07/24 00:00
PHST- 1997/07/24 00:00 [pubmed]
PHST- 1997/07/24 00:01 [medline]
PHST- 1997/07/24 00:00 [entrez]
AID - 10.1038/sj.onc.1201194 [doi]
PST - ppublish
SO  - Oncogene. 1997 Jul 24;15(4):417-22. doi: 10.1038/sj.onc.1201194.