PMID- 9244454
OWN - NLM
STAT- MEDLINE
DCOM- 19971020
LR  - 20191102
IS  - 0967-3849 (Print)
IS  - 0967-3849 (Linking)
VI  - 5
IP  - 4
DP  - 1997 Jun
TI  - Fluorescence in situ hybridization of ribosomal DNA to mitotic chromosomes of 
      tsetse flies (Diptera: Glossinidae: Glossina).
PG  - 262-7
AB  - Ribosomal genes were mapped in Glossina austeni, G. brevipalpis, G. f. fuscipes, 
      G. m. submorsitans, G. p. palpalis, G. pallidipes and G. tachinoides on mitotic 
      chromosomes by fluorescence in situ hybridization (FISH) using Drosophila hydei 
      genomic clones that contain the 28S ribosomal DNA. In all species except G. 
      brevipalpis, the ribosomal genes were located on the long arm of autosome L1. The 
      Y chromosomes of G. pallidipes and G. p. palpalis showed additional hybridization 
      signals. Supernumerary chromosomes were found in G. austeni, G. brevipalpis and 
      G. pallidipes. The C-banding pattern obtained by in situ hybridization was 
      compared with Giemsa C-banding patterns that were published previously. The 
      karyotype of G. brevipalpis was found to differ from that of other Glossina 
      species, with either two or three FISH signals being obtained with a ribosomal 
      probe, depending on the individual analysed. The rDNA genes are the first 
      physically mapped markers in tsetse flies and will be useful for mapping 
      approaches.
FAU - Willhoeft, U
AU  - Willhoeft U
AD  - FAO/IAEA (Food Agriculture Organization/International Atomic Energy Agency) 
      Agricultural and Biotechnology Laboratory, Seibersdorf, Austria. 
      ute_willhoeft@magicvillage.de
LA  - eng
PT  - Comparative Study
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - Netherlands
TA  - Chromosome Res
JT  - Chromosome research : an international journal on the molecular, supramolecular 
      and evolutionary aspects of chromosome biology
JID - 9313452
RN  - 0 (DNA Probes)
RN  - 0 (DNA, Ribosomal)
SB  - IM
MH  - Animals
MH  - Chromosome Banding
MH  - Chromosome Mapping/*methods
MH  - DNA Probes
MH  - DNA, Ribosomal/*genetics
MH  - Female
MH  - In Situ Hybridization, Fluorescence/*methods
MH  - Karyotyping
MH  - Mitosis
MH  - Repetitive Sequences, Nucleic Acid
MH  - Species Specificity
MH  - Tsetse Flies/*genetics
MH  - Y Chromosome/genetics
EDAT- 1997/06/01 00:00
MHDA- 1997/06/01 00:01
CRDT- 1997/06/01 00:00
PHST- 1997/06/01 00:00 [pubmed]
PHST- 1997/06/01 00:01 [medline]
PHST- 1997/06/01 00:00 [entrez]
AID - 10.1023/a:1018471620542 [doi]
PST - ppublish
SO  - Chromosome Res. 1997 Jun;5(4):262-7. doi: 10.1023/a:1018471620542.