PMID- 9245350
OWN - NLM
STAT- PubMed-not-MEDLINE
LR  - 20191120
IS  - 1095-9130 (Electronic)
IS  - 1046-2023 (Linking)
VI  - 9
IP  - 1
DP  - 1996 Feb
TI  - Gene Copy Number Analysis by Fluorescence in Situ Hybridization and Comparative 
      Genomic Hybridization.
PG  - 113-21
AB  - Fluorescence in situ hybridization (FISH) with gene- and locus-specific probes 
      provides a rapid means to assess copy numbers of specific sequences in individual 
      interphase nuclei. Recent technical improvements have made FISH applicable to the 
      analysis of both fresh and archival tissue specimens in research as well as in 
      diagnostic laboratories. FISH is limited to analysis of one or a few loci at a 
      time, making genome-wide surveys impractical. Comparative genomic hybridization 
      (CGH) was developed as a means to screen entire genomes for DNA sequence copy 
      number changes. CGH is based on the cohybridization of differentially labeled 
      test and reference DNAs to normal metaphase chromosomes. Measurement of the test 
      to reference fluorescence ratios along all chromosomes provides information on 
      chromosomal regions that are over- or underrepresented in the test genome. The 
      use of these two techniques will be illustrated in the analysis of genetic 
      changes in solid tumors. The techniques are complementary to one another and have 
      proven to be highly useful for identification of previously unknown genetic 
      changes and genes that play an important role in tumor progression.
FAU - Kallioniemi, A
AU  - Kallioniemi A
AD  - Laboratory of Cancer Genetics, Tampere University Hospital, Tampere, Finland
FAU - Visakorpi, T
AU  - Visakorpi T
FAU - Karhu, R
AU  - Karhu R
FAU - Pinkel, D
AU  - Pinkel D
FAU - Kallioniemi, OP
AU  - Kallioniemi OP
LA  - eng
PT  - Journal Article
PL  - United States
TA  - Methods
JT  - Methods (San Diego, Calif.)
JID - 9426302
EDAT- 1996/02/01 00:00
MHDA- 1996/02/01 00:01
CRDT- 1996/02/01 00:00
PHST- 1996/02/01 00:00 [pubmed]
PHST- 1996/02/01 00:01 [medline]
PHST- 1996/02/01 00:00 [entrez]
AID - S1046202396900158 [pii]
AID - 10.1006/meth.1996.0015 [doi]
PST - ppublish
SO  - Methods. 1996 Feb;9(1):113-21. doi: 10.1006/meth.1996.0015.